An enhanced ascorbate peroxidase 2/antibody-binding domain fusion protein (APEX2-ABD) as a recombinant target-specific signal amplifier

Autor:	Junseon Min, Hyun-Woo Rhee, Jisu Lee, Eun Kyung Song, Tae Joo Park, Moonil Kim, Sebyung Kang, Yoonji Bae
Rok vydání:	2015
Předmět:	Circular dichroism Recombinant Fusion Proteins Catalysis Antibodies law.invention Mice Protein structure Ascorbate Peroxidases law Cell Line Tumor Materials Chemistry Animals Surface plasmon resonance Fluorescent Dyes biology Chemistry Circular Dichroism fungi Metals and Alloys General Chemistry Hydrogen Peroxide Surface Plasmon Resonance Primary and secondary antibodies Fusion protein Molecular biology Surfaces Coatings and Films Electronic Optical and Magnetic Materials Protein Structure Tertiary Biochemistry Microscopy Fluorescence Cell culture Immunoglobulin G Ceramics and Composites biology.protein Recombinant DNA Quartz Crystal Microbalance Techniques Peroxidase
Zdroj:	Chemical communications (Cambridge, England). 51(54)
ISSN:	1364-548X
Popis:	A recombinant target-specific signal amplifier was constructed by genetically fusing the enhanced ascorbate peroxidase 2 (APEX2) and an antibody-binding domain (ABD). The fusion protein APEX2–ABD possessed the peroxidase activity and the antibody-binding capability simultaneously and replaced the conventional HRP-conjugated secondary antibodies in a TSA assay for amplifying fluorescence signals.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9c91c964e41944cc084c4741dd66db4c https://pubmed.ncbi.nlm.nih.gov/26063640 Zobrazit plný text záznamu