Secondary Metabolites in Allergic Plant Pollen Samples Modulate Afferent Neurons and Murine Tracheal Rings

Autor: C Nassenstein, Silke Wiegand, Alen Božičević, Matthias Hamburger, Maria De Mieri
Rok vydání: 2017
Předmět:
0301 basic medicine
Time Factors
Pharmaceutical Science
Asteraceae
medicine.disease_cause
Sesquiterpene
01 natural sciences
High-performance liquid chromatography
Afferent Neurons
Analytical Chemistry
Mice
03 medical and health sciences
chemistry.chemical_compound
Pollen
Drug Discovery
Botany
otorhinolaryngologic diseases
medicine
Animals
Humans
Neurons
Afferent
Nuclear Magnetic Resonance
Biomolecular
Chromatography
High Pressure Liquid
Ambrosia artemisiifolia
Pharmacology
Molecular Structure
biology
010405 organic chemistry
Organic Chemistry
food and beverages
Allergens
biology.organism_classification
Acetylcysteine
0104 chemical sciences
Trachea
030104 developmental biology
Complementary and alternative medicine
Biochemistry
chemistry
Molecular Medicine
Ambrosia
Polyamine
Sesquiterpenes
Intracellular
Zdroj: Journal of Natural Products. 80:2953-2961
ISSN: 1520-6025
0163-3864
DOI: 10.1021/acs.jnatprod.7b00495
Popis: Plant pollens are strong airborne elicitors of asthma. Their proteinaceous allergens have been studied intensively, but little is known about a possible contribution of pollen secondary metabolites to the nonallergic exacerbation of asthma. Pollen samples originating from 30 plant species were analyzed by HPLC coupled to PDA, ESIMS, and ELSD detectors and off-line NMR spectroscopy. Polyamine conjugates, flavonoids, and sesquiterpene lactones were identified. Polyamine conjugates were characteristic of all Asteraceae species. The presence of sesquiterpene lactones in Asteraceae pollen varied between species and pollen lots. All plant pollen, including those from non-Asteraceae species, contained to some extent electrophiles as determined by their reaction with N-acetyl-l-cysteine. Selected pollen extracts and pure compounds were tested in murine afferent neurons and in murine tracheal preparations. Tetrahydrofuran extracts of Ambrosia artemisiifolia and Ambrosia psilostachya pollen and a mixture of sesquiterpene lactones coronopilin/parthenin increased the intracellular Ca2+ concentration in 15%, 32%, and 37% of cinnamaldehyde-responsive neurons, respectively. In organ bath experiments, only the sesquiterpene lactones tested induced a weak dilatation of naive tracheas and strongly lowered the maximal methacholine-induced tracheal constriction. A tetrahydrofuran extract of A. psilostachya and coronopilin/parthenin led to a time-dependent relaxation of the methacholine-preconstricted trachea. These results provide the first evidence for a potential role of pollen secondary metabolites in the modulation of the tracheal tone.
Databáze: OpenAIRE
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