CD36 and SR-BI Are Involved in Cellular Uptake of Provitamin A Carotenoids by Caco-2 and HEK Cells, and Some of Their Genetic Variants Are Associated with Plasma Concentrations of These Micronutrients in Humans

Autor: Sophie Lecompte, Anne Marie Minihane, Myriam Moussa, Aurélie Goncalves, Marcela González Gross, John C. Mathers, Philip C. Calder, Pilar Galan, Fabien Szabo de Edelenyi, Peter J. Curtis, Elizabeth A. Miles, Chris J. Packard, Christina Breidenassel, Franck Tourniaire, Aline Meirhaeghe, Serge Hercberg, Louisa Goumidi, Georg Lietz, Emmanuelle Kesse-Guyot, Patrick Borel, Muriel J. Caslake, Emmanuelle Reboul
Přispěvatelé: Nutrition, obésité et risque thrombotique (NORT), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Newcastle University [Newcastle], Equipe 3: EREN- Equipe de Recherche en Epidémiologie Nutritionnelle (CRESS - U1153), Université Paris 13 (UP13)-Institut National de la Recherche Agronomique (INRA)-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Epidémiologie des maladies chroniques : impact des interactions gène environnement sur la santé des populations, Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Droit et Santé, Division of Cardiovascular and Medical Sciences, Glasgow Royal Infirmary, UK, Glasgow Royal Infirmary, Division of Cardiovascular and Medical Sciences, University of Glasgow, Information, Evidence and Research Cluster, Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Nutriments Lipidiques et Prévention des Maladies Métaboliques, Université de la Méditerranée - Aix-Marseille 2-Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), University of Southampton, Funding Agency and Grant Number : Direction Generale de la Sante (Ministry of Health), Mederic, Ipsen, Mutuelle Generale de l'Education Nationale, Sodexho, Pierre Fabre, Agence Nationale de la Recherche [ANR-05-PNRA-010], European Union [FOOD-CT-2005-007034], Universidad Politecnica de Madrid [CH/018/2008], Axis-Shield Diagnostics, Ltd. (Oslo, Norway), Abbot Cientifica S.A. (Spain), Spanish Ministry of Education [EX-2007-1124], Cognis GmbH (Germany), UK Food Standards Agency [RRD7/N02/A], Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)-Université de la Méditerranée - Aix-Marseille 2, Institut National de la Recherche Agronomique (INRA)-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Université Paris 13 (UP13)-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Borel, Patrick, Université Paris 13 (UP13)-Institut National de la Recherche Agronomique (INRA)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Nutrition, obésité et risque thrombotique ( NORT ), Institut National de la Recherche Agronomique ( INRA ) -Aix Marseille Université ( AMU ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Equipe 3: EREN- Equipe de Recherche en Epidémiologie Nutritionnelle ( CRESS - U1153 ), Université Paris 13 ( UP13 ) -Institut National de la Recherche Agronomique ( INRA ) -Conservatoire National des Arts et Métiers [CNAM] ( CNAM ) -Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité ( CRESS (U1153 / UMR_A 1125) ), Institut National de la Recherche Agronomique ( INRA ) -Université Sorbonne Paris Cité ( USPC ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Institut National de la Recherche Agronomique ( INRA ) -Université Sorbonne Paris Cité ( USPC ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Réseau International des Instituts Pasteur ( RIIP ) -Réseau International des Instituts Pasteur ( RIIP ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université de Lille, Droit et Santé, WHO(OMS)
Jazyk: angličtina
Rok vydání: 2013
Předmět:
CD36 Antigens
Male
030309 nutrition & dietetics
[ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition
CD36
INTESTINAL-ABSORPTION
Medicine (miscellaneous)
Xanthophylls
Intestinal absorption
chemistry.chemical_compound
GOLDEN RICE
Vitamin A
Carotenoid
Cryptoxanthins
IN-VIVO
ComputingMilieux_MISCELLANEOUS
chemistry.chemical_classification
0303 health sciences
Nutrition and Dietetics
biology
Dioxygenase activity
Retinol
food and beverages
Scavenger Receptors
Class B
beta Carotene
Biochemistry
Female
EXPRESSION
DIOXYGENASE ACTIVITY
Adolescent
Genotype
LINE
METABOLISM
Polymorphism
Single Nucleotide
03 medical and health sciences
FAT-SOLUBLE VITAMINS
Sex Factors
beta-Carotene
A-DEFICIENCY
Humans
Scavenger receptor
030304 developmental biology
Genetic Variation
Carotenoids
SCARB1
TRANSPORT
[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition
Cross-Sectional Studies
HEK293 Cells
chemistry
biology.protein
Caco-2 Cells
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Zdroj: Journal of Nutrition
Journal of Nutrition, American Society for Nutrition, 2013, 143 (4), pp.448-456. ⟨10.3945/jn.112.172734⟩
Journal of Nutrition, 2013, 143 (4), pp.448-456. ⟨10.3945/jn.112.172734⟩
Journal of Nutrition, American Society for Nutrition, 2013, 143 (4), pp.448-456. 〈10.3945/jn.112.172734〉
ISSN: 0022-3166
Popis: International audience; Scavenger receptor class B type I (SR-BI) and cluster determinant 36 (CD36) have been involved in cellular uptake of some provitamin A carotenoids. However, data are incomplete (e.g., there are no data on a-carotene), and it is not known whether genetic variants in their encoding genes can affect provitamin A carotenoid status. The objectives were 1) to assess the involvement of these scavenger receptors in cellular uptake of the main provitamin A carotenoids (i.e., beta-carotene, alpha-carotene, and beta-cryptoxanthin) as well as that of preformed vitamin A (i.e., retinol) and 2) to investigate the contribution of genetic variations in genes encoding these proteins to interindividual variations in plasma concentrations of provitamin A carotenoids. The involvement of SR-BI and CD36 in carotenoids and retinol cellular uptake was investigated in Caco-2 and human embryonic kidney (HEK) cell lines. The involvement of scavenger receptor class B type I (SCARB1) and CD36 genetic variants on plasma concentrations of provitamin A carotenoids was assessed by association studies in 3 independent populations. Cell experiments suggested the involvement of both proteins in cellular uptake of provitamin A carotenoids but not in that of retinol. Association studies showed that several plasma provitamin A carotenoid concentrations were significantly different (P < 0.0083) between participants who bore different genotypes at single nucleotide polymorphisms and haplotypes in CD36 and SCARB1. In conclusion, SR-BI and CD36 are involved in cellular uptake of provitamin A carotenoids, and genetic variations in their encoding genes may modulate plasma concentrations of provitamin A carotenoids at a population level. J. Nutr. 143: 448-456, 2013.
Databáze: OpenAIRE
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