Metformin inhibits cholesterol‑induced adhesion molecule expression via activating the AMPK signaling pathway in vascular smooth muscle cells
| Autor: | Jingbo Hou, Lu Zhang, Xuedong Wang, Xingtao Huang, Mengyue Yang, Wenjuan Du, Qi Liu, Ruoxi Zhang |
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| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
Vascular smooth muscle p38 mitogen-activated protein kinases Myocytes Smooth Muscle Vascular Cell Adhesion Molecule-1 AMP-Activated Protein Kinases p38 Mitogen-Activated Protein Kinases Biochemistry Monocytes Muscle Smooth Vascular Genetics Humans Protein kinase A Cell adhesion Molecular Biology AMP-activated protein kinase signaling pathway Cell adhesion molecule Chemistry NF-kappa B Transcription Factor RelA cholesterol AMPK Articles Adhesion Atherosclerosis Intercellular Adhesion Molecule-1 adhesion molecule Metformin Cell biology Oncology Molecular Medicine Signal transduction Reactive Oxygen Species Cell Adhesion Molecules Signal Transduction |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2021.12348 |
| Popis: | Recruitment of lymphocytes to the vascular wall contributes to the pathogenesis of atherosclerosis (AS). The expression of cellular adhesion molecules, such as vascular cell adhesion molecule‑1 and intercellular adhesion molecule‑1, serves a critical role in mediating lymphocyte adhesion to the vascular wall. Cholesterol loading induces the expression of adhesion molecules in vascular smooth muscle cells (VSMCs), but the underlying mechanism is not completely understood. The present study aimed to investigate the mechanism underlying the effects of cholesterol on adhesion molecule expression, and whether metformin protected VSMCs against cholesterol‑induced functional alterations. Human VSMCs were loaded with cholesterol and different concentrations of metformin. The expression levels of adhesion molecules were assessed via reverse transcription‑quantitative PCR and western blotting. Reactive oxygen species (ROS) accumulation and levels were quantified via fluorescence assays and spectrophotometry, respectively. AMP‑activated protein kinase (AMPK), p38 MAPK and NF‑κB signaling pathway‑related protein expression levels were evaluated via western blotting. Compared with the control group, cholesterol loading significantly upregulated adhesion molecule expression levels on VSMCs by increasing intracellular ROS levels and activating the p38 MAPK and NF‑κB signaling pathways. Metformin decreased cholesterol‑induced VSMC damage by activating the AMPK signaling pathway, and suppressing p38 MAPK and NF‑κB signaling. The present study indicated the therapeutic potential of metformin for VSMC protection, reduction of monocyte adhesion, and ultimately, the prevention and treatment of AS. |
| Databáze: | OpenAIRE |
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