Myo-Inositol as a carbon substrate in Francisella and insights into the metabolism of Francisella sp. strain W12-1067
| Autor: | Kristin Köppen, Fan Chen, Wolfgang Eisenreich, Klaus Heuner, Rosa Einenkel, Duc Tung Vu, Clara Morguet, Kerstin Rydzewski |
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| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
Genomic Islands Mutant Pentose phosphate pathway Microbiology Inositol oxygenase Francisella sp. W12-1067 03 medical and health sciences Other systems of medicine Isotopolog profiling Gene cluster Allofrancisella Computer Simulation Amino Acids Francisella 030304 developmental biology 0303 health sciences biology Strain (chemistry) 030306 microbiology Inositol Oxygenase General Medicine Metabolism biology.organism_classification Pathogenicity island QR1-502 Carbon ddc Infectious Diseases Glucose Biochemistry Multigene Family Myo-inositol Water Microbiology RZ201-999 Genome Bacterial Inositol |
| Zdroj: | International Journal of Medical Microbiology, Vol 310, Iss 4, Pp 151426-(2020) |
| ISSN: | 1618-0607 |
| Popis: | Recently, a new environmental Francisella strain, Francisella sp. strain W12-1067, has been identified in Germany. This strain is negative for the Francisella pathogenicity island (FPI) but exhibits a putative alternative type VI secretion system. Some known virulence factors of Francisella are present, but the pathogenic capacity of this species is not known yet. In silico genome analysis reveals the presence of a gene cluster tentatively enabling myo-inositol (MI) utilization via a putative inositol oxygenase. Labelling experiments starting from 2H-inositol demonstrate that this gene cluster is indeed involved in the metabolism of MI. We further show that, under in vitro conditions, supply of MI increases growth rates of strain W12-1067 in the absence of glucose and that the metabolism of MI is strongly reduced in a W12-1067 mutant lacking the MI gene cluster. The positive growth effect of MI in the absence of glucose is restored in this mutant strain by introducing the complete MI gene cluster. F. novicida Fx1 is also positive for the MI metabolizing gene cluster and MI again increases growth in a glucose-free medium, in contrast to F. novicida strain U112, which is shown to be a natural mutant of the MI metabolizing gene cluster. Labelling experiments of Francisella sp. strain W12-1067 in medium T containing 13C-glucose, 13C-serine or 13C-glycerol as tracers suggest a bipartite metabolism where glucose is mainly metabolized through glycolysis, but not through the Entner-Doudoroff pathway or the pentose phosphate pathway. Carbon flux from 13C-glycerol and 13C-serine is less active, and label from these tracers is transferred mostly into amino acids, lactate and fatty acids. Together, the metabolism of Francisella sp. strain W12-1067 seems to be more related to the respective one in F. novicida rather than in F. tularensis subsp. holarctica. |
| Databáze: | OpenAIRE |
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