Stereological analysis of bacterial load and lung lesions in nonhuman primates (rhesus macaques) experimentally infected with Mycobacterium tuberculosis
| Autor: | Karen L. Oslund, Dallas M. Hyde, Heather Offenstein, Nicholas W. Lerche, Laurie L. Brignolo, Don R. Canfield, Linda Hirst, Paul A. Luciw, Erik R. Wisner, Xiao Wei Yang, Frank Ventimiglia, Lourdes Adamson, Miles Christensen, David M. Lewinsohn, Ross P. Tarara, Resmi Ravindran |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Pulmonary and Respiratory Medicine
Male Pathology medicine.medical_specialty Tuberculosis Granuloma Respiratory Tract Physiology H&E stain Stereology Severity of Illness Index Mycobacterium tuberculosis Physiology (medical) Bronchoscopy medicine Intubation Intratracheal Animals Humans Pulmonary pathology Hematoxylin Pathogen Lung Tuberculosis Pulmonary Microscopy biology Tissue Extracts Cell Biology Articles Organ Size medicine.disease biology.organism_classification Macaca mulatta Bacterial Load Disease Models Animal medicine.anatomical_structure Granuloma Immunology Eosine Yellowish-(YS) |
| Popis: | Infection with Mycobacterium tuberculosis primarily produces a multifocal distribution of pulmonary granulomas in which the pathogen resides. Accordingly, quantitative assessment of the bacterial load and pathology is a substantial challenge in tuberculosis. Such assessments are critical for studies of the pathogenesis and for the development of vaccines and drugs in animal models of experimental M. tuberculosis infection. Stereology enables unbiased quantitation of three-dimensional objects from two-dimensional sections and thus is suited to quantify histological lesions. We have developed a protocol for stereological analysis of the lung in rhesus macaques inoculated with a pathogenic clinical strain of M. tuberculosis (Erdman strain). These animals exhibit a pattern of infection and tuberculosis similar to that of naturally infected humans. Conditions were optimized for collecting lung samples in a nonbiased, random manner. Bacterial load in these samples was assessed by a standard plating assay, and granulomas were graded and enumerated microscopically. Stereological analysis provided quantitative data that supported a significant correlation between bacterial load and lung granulomas. Thus this stereological approach enables a quantitative, statistically valid analysis of the impact of M. tuberculosis infection in the lung and will serve as an essential tool for objectively comparing the efficacy of drugs and vaccines. |
| Databáze: | OpenAIRE |
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