Recapitulating early development of mouse musculoskeletal precursors of the paraxial mesoderm in vitro
| Autor: | Ziad Al Tanoury, Shahragim Tajbakhsh, Barbara Gayraud-Morel, Philippe Moncuquet, Jérome Chal, Jean-Marie Garnier, Olivier Pourquié, Olivier Tassy, Agata Bera, Olga Sumara, Masayuki Oginuma, Alexis Hubaud, Getzabel Guevara, Leif Kennedy, Marie Knockaert, Ayako Miyanari, Bénédicte Gobert |
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| Přispěvatelé: | Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Brigham & Women’s Hospital [Boston] (BWH), Harvard Medical School [Boston] (HMS), Harvard Stem Cell Institute [Cambridge, USA] (HSCI), Harvard University, Anagenesis Biotechnologies [Illkirch Graffenstaden], Cellules Souches et Développement / Stem Cells and Development, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), This work was supported by an advanced grant from the European Research Council (ERC-2009-AdG 249931 to O.P.), by a Seventh Framework Programme grant (Plurimes 602423) and by a strategic project from the Association Française contre les Myopathies (AFM-Téléthon) to O.P, We thank Christopher Henderson for critical reading of the manuscript. We are grateful to Jennifer Pace and Tania Knauer-Meyer for their help, and to Laurent Bianchetti for Bioinformatic support. We thank the cytometry (Claudine Ebel), microarray (Christelle Thibault-Carpentier) and cell culture facilities at the IGBMC, and the Cytometry and Animal Facilities at the Pasteur Institute for assistance, European Project: 249931,EC:FP7:ERC,ERC-2009-AdG,BODYBUILT(2010), European Project: 602423,EC:FP7:HEALTH,FP7-HEALTH-2013-INNOVATION-1,PLURIMES(2014), Harvard University [Cambridge], Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Gayraud-Morel, Barbara, Building The Vertebrate Body - BODYBUILT - - EC:FP7:ERC2010-04-01 - 2015-03-31 - 249931 - VALID, Pluripotent stem cell resources for mesodermal medicine - PLURIMES - - EC:FP7:HEALTH2014-02-01 - 2018-01-31 - 602423 - VALID |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cellular differentiation MESH: Bone Morphogenetic Proteins [SDV]Life Sciences [q-bio] PAX3 Skeletal muscle MESH: Flow Cytometry [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] Fibroblast growth factor Muscle Development Mesoderm Mice 0302 clinical medicine MESH: Gene Expression Regulation Developmental [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] MESH: Animals Induced pluripotent stem cell Wnt Signaling Pathway [SDV.BDD]Life Sciences [q-bio]/Development Biology In Situ Hybridization MESH: Mesoderm MESH: Muscle Skeletal Myogenesis MESH: Real-Time Polymerase Chain Reaction Wnt signaling pathway MESH: Wnt Signaling Pathway Gene Expression Regulation Developmental Cell Differentiation Flow Cytometry Immunohistochemistry Cell biology [SDV] Life Sciences [q-bio] Paraxial mesoderm Bone Morphogenetic Proteins MESH: Muscle Development MESH: Pluripotent Stem Cells Presomitic mesoderm MESH: Tissue Array Analysis MESH: Cell Differentiation MESH: Immunophenotyping Bioengineering [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology In Vitro Techniques Real-Time Polymerase Chain Reaction Immunophenotyping 03 medical and health sciences MESH: Gene Expression Profiling MESH: In Situ Hybridization Pluripotent stem cells [SDV.BDD] Life Sciences [q-bio]/Development Biology Animals Humans Muscle Skeletal Molecular Biology [SDV.BC] Life Sciences [q-bio]/Cellular Biology MESH: Mice MESH: In Vitro Techniques MESH: Humans Lateral plate mesoderm Gene Expression Profiling fungi MESH: Immunohistochemistry 030104 developmental biology Tissue Array Analysis Satellite cell 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Development (Cambridge, England) Development (Cambridge, England), 2018, 145 (6), pp.dev157339. ⟨10.1242/dev.157339⟩ Development (Cambridge, England), Company of Biologists, 2018, 145 (6), pp.dev157339. ⟨10.1242/dev.157339⟩ |
| ISSN: | 0950-1991 1477-9129 |
| Popis: | The work described in this article is partially covered by patent application PCT/EP2012/066793 (publication number WO2013030243 A1). O.P., J.C. and M.K. are co-founders and shareholders of Anagenesis Biotechnologies, a start-up company specializing in the production of muscle cells in vitro for cell therapy and drug screenin; International audience; Body skeletal muscles derive from the paraxial mesoderm, which forms in the posterior region of the embryo. Using microarrays, we characterize novel mouse presomitic mesoderm (PSM) markers and show that, unlike the abrupt transcriptome reorganization of the PSM, neural tube differentiation is accompanied by progressive transcriptome changes. The early paraxial mesoderm differentiation stages can be efficiently recapitulated in vitro using mouse and human pluripotent stem cells. While Wnt activation alone can induce posterior PSM markers, acquisition of a committed PSM fate and efficient differentiation into anterior PSM Pax3+ identity further requires BMP inhibition to prevent progenitors from drifting to a lateral plate mesoderm fate. When transplanted into injured adult muscle, these precursors generated large numbers of immature muscle fibers. Furthermore, exposing these mouse PSM-like cells to a brief FGF inhibition step followed by culture in horse serum-containing medium allows efficient recapitulation of the myogenic program to generate myotubes and associated Pax7+ cells. This protocol results in improved in vitro differentiation and maturation of mouse muscle fibers over serum-free protocols and enables the study of myogenic cell fusion and satellite cell differentiation. |
| Databáze: | OpenAIRE |
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