Association of interleukin-17A gene polymorphisms and susceptibility to systemic lupus erythematosus in Egyptian children and adolescents: a multi-centre study
| Autor: | Mohamed A Elkoumi, Mayy AN Allah, Faisal Y Mohamed, Naglaa F Boraey, Sawsan H Abdellatif, Mohamed MM Shehab, Ahmed H Sherif, Nagwa E Akeel, Rabab M Saleh, Anas M Elshreif, Hind M Abdelrahman, Attia A Soliman, Ahmed A Emam, Manal AA Youssef, Dalia S Fahmy, Mohammad M Sallam, Abdalla M Nawara, Elsayed A Elgohary, Ali Ismael, Sameh MH El-Kaffas, Alaa A Sobeih, Lamya M Ibrahim, Mohamed AM Ibrahim, Adel M Abdou, Sherif M Yousry, Sherif F Osman, Fatma M El-Deeb, Ahmed A Elhewala, Sahbaa FM Hafez, Nevin M Waked, Hany AA Elbasyouni, Rania A Fouad, Nancy MS Zeidan, Mohamed Nashat, Mohsen AA Farghaly |
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| Rok vydání: | 2020 |
| Předmět: |
030203 arthritis & rheumatology
0301 basic medicine Male Adolescent Interleukin-17 Lupus Nephritis Polymorphism Single Nucleotide 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Logistic Models Rheumatology Gene Frequency Risk Factors Case-Control Studies Humans Lupus Erythematosus Systemic Egypt Female Genetic Predisposition to Disease Prospective Studies Child Alleles |
| Zdroj: | Lupus. 29(7) |
| ISSN: | 1477-0962 |
| Popis: | BackgroundRecently, the interleukin-17A ( IL-17A) gene has emerged as a potential candidate gene for autoimmune disorders, including systemic lupus erythematosus (SLE).ObjectivesThis study aimed to investigate whether IL-17A polymorphisms at rs2275913 G/A, rs8193036 C/T and rs3748067 C/T could be susceptibility markers for juvenile-onset SLE (JSLE) and lupus nephritis (LN) in Egyptian children and adolescents.MethodsIn this multi-centre study, we genotyped 320 patients diagnosed with JSLE and 320 matched control children for three IL-17A polymorphisms at rs2275913 G/A, rs8193036 C/T and rs3748067 C/T using TaqMan probe-based real-time polymerase chain reaction. Meanwhile, IL-17A serum levels were assessed using ELISA.ResultsThe IL-17 rs2275913 A/A genotype and A allele were more represented in JSLE patients compared to the control group (21% vs. 7%, odds ratio (OR) = 3.8, 95% confidence interval (CI) 1.78–5.5, p = 0.001, pBonf = 0.003 for the A/A genotype; 37% vs. 29%, OR = 1.4, 95% CI 1.11–1.8, p = 0.003, pBonf = 0.009 for the A allele. No significant difference was found for IL-17 rs8193036 and rs3748067 single nucleotide polymorphisms (SNPs) in genotype distribution or allele frequencies ( p>0.05). Patients carrying the IL-17 rs2275913 A/A genotype and A allele were more likely to develop LN (OR = 5.64, 95% CI 2.39–13.77, pBonf = 0.001 for the A/A genotype; OR = 2.73, 95% CI 1.84–4.07, pBonf = 0.02 for the A allele).ConclusionThe IL-17 rs2275913 A allele and A/A genotype were associated with high IL-17 serum levels and may contribute to susceptibility to JSLE and the development of LN in Egyptian children and adolescents. However, no significant association was evident between the studied IL-17A SNPs and other clinical phenotypes, disease activity scores or laboratory profile of JSLE. |
| Databáze: | OpenAIRE |
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