Differentiation Potential of Pancreatic Fibroblastoid Cells/Stellate Cells: Effects of Peroxisome Proliferator-Activated Receptor Gamma Ligands
| Autor: | ML Kruse, A. Schmid, H Schäfer, Alexander Arlt, C. Timke, B. Agricola, S. Hopf-Jensen, G. Sparmann, Bence Sipos |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
chemistry.chemical_classification
medicine.medical_specialty Pancreatic disease Article Subject lcsh:Cytology business.industry Peroxisome proliferator-activated receptor Lipid metabolism Cell Biology medicine.disease In vitro Cell biology chemistry.chemical_compound Endocrinology chemistry Adipocyte Ciglitazone Internal medicine medicine Hepatic stellate cell lcsh:QH573-671 Receptor business Research Article |
| Zdroj: | International Journal of Cell Biology International Journal of Cell Biology, Vol 2011 (2011) |
| ISSN: | 1687-8876 |
| DOI: | 10.1155/2011/816791 |
| Popis: | Pancreatic stellate cells have been investigated mostly for their activation process, supposed to support the development of pancreatic disease. Few studies have been presented on reversal of the activation process in vitro. Thiazolidinediones (TZDs) have been used as antidiabetics and have now been reported to exert antifibrotic activity. We tested effects of natural and synthetic ligands of peroxisome proliferator-activated receptor gamma (PPARγ) on human pancreatic fibroblastoid cells (hPFCs) in search for specificity of action. Ciglitazone, as a prototype of TZDs, was shown to have reversible growth inhibitory effects on human pancreatic fibroblastoid cells/stellate cells. Cells treated with ciglitazone for three days showed enhanced lipid content and induction of proteins involved in lipid metabolism. Collagen synthesis was reduced in hPFC. Interaction of PPARγwith DNA binding sites upon ligand binding was shown by gel shift analysis. These findings point toward a potential for adipocyte differentiation in human pancreatic fibroblastoid cells. |
| Databáze: | OpenAIRE |
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