Effect of dietary supplementation with vitamin E and stocking density on macrophage recruitment and giant cell formation in the teleost fish, Piaractus mesopotamicus
| Autor: | Flávio Ruas de Moraes, Vando Edésio Soares, J. E. R. Moraes, Marco Antonio de Andrade Belo, Alda Maria Machado Bueno Otoboni, Sérgio Henrique Canello Schalch |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Blood Glucose
Giant Cells Foreign-Body medicine.medical_specialty food.ingredient Hydrocortisone medicine.medical_treatment Cell Count Carbohydrate metabolism Biology Pathology and Forensic Medicine Piaractus mesopotamicus chemistry.chemical_compound Stocking food Cell Movement Internal medicine medicine Animals Vitamin E Animal Husbandry Population Density General Veterinary Dose-Response Relationship Drug Foreign-Body Reaction Macrophages Fishes Tilapia biology.organism_classification Diet Endocrinology Crowding chemistry Giant cell Langerhans Cells Tocopheryl acetate alpha-Tocopherol |
| Zdroj: | Journal of comparative pathology. 133(2-3) |
| ISSN: | 0021-9975 |
| Popis: | The effect of dietary supplementation with 0, 100 and 450 mg of vitamin E (DL-alpha tocopheryl acetate)/kg of a dry diet on the kinetics of macrophage recruitment and giant cell formation in the pacu, maintained at different stocking densities (5 kg/m(3) and 20 kg/m(3)), was investigated by insertion of round glass coverslips into the subcutaneous connective tissue. After a feeding period of 18 weeks, the coverslips were implanted and later removed for examination at 2, 7 and 15 days post-implantation. Fish fed diets supplemented with 450 mg of vitamin E showed an increase (P0.05) in the accumulation of macrophages, foreign body giant cells and Langhans type cells. The kinetics of macrophage recruitment and giant cell formation on the glass coverslips appeared to be strongly influenced by vitamin E supplementation, since fish fed a basal diet and held at high stocking densities showed low numbers of adhering cells on the coverslips, and high concentrations of plasma corticosteroids. On the other hand, fish given a diet supplemented with 450 mg of vitamin E did not show a similar difference in plasma cortisol concentrations related to stocking density. The effect of cortisol concentrations on carbohydrate metabolism, analysed by assessment of plasma glycaemia, was not clear. Blood glucose concentrations did not vary substantially with the different treatments examined. These results suggest that vitamin E may contribute to the efficiency of the fish's inflammatory response by increasing macrophage recruitment and giant cell formation in the foreign body granulomatous reaction. Vitamin E appeared to act on the stress response of pacus by preventing a stress-related immunosuppression. |
| Databáze: | OpenAIRE |
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