Transformation of ciprofloxacin-resistant Neisseria gonorrhoeae gyrA, parE and porB1b genes
| Autor: | Bengt Wretlind, Magnus Unemo, Sohidul Islam, Emma Lindbäck, Camilla Lang |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
DNA Topoisomerase IV
Microbiology (medical) Sexually transmitted disease Porins Microbial Sensitivity Tests medicine.disease_cause DNA gyrase Microbiology Minimum inhibitory concentration Ciprofloxacin Drug Resistance Bacterial medicine Pharmacology (medical) Antibacterial agent biology General Medicine biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Neisseria gonorrhoeae Anti-Bacterial Agents Transformation (genetics) Infectious Diseases DNA Gyrase Neisseriaceae Transformation Bacterial medicine.drug |
| Zdroj: | International Journal of Antimicrobial Agents. 28:206-211 |
| ISSN: | 0924-8579 |
| DOI: | 10.1016/j.ijantimicag.2006.04.003 |
| Popis: | In several transformation experiments, we have shown that introduction of an alteration in GyrA at position 95 of a ciprofloxacin-susceptible Neisseria gonorrhoeae strain (minimum inhibitory concentration (MIC) 0.008 mg/L) increases the MIC to 0.064 mg/L. Two alterations (positions 91 and 95) increase the MIC to 0.125-0.25 mg/L. Transformants with ciprofloxacin MICs of 0.5-16 mg/L were obtained from a moderately ciprofloxacin-resistant strain (MIC 0.25 mg/L). These transformants had alterations in the gene for PorB1b and probably other genes. In one transformant, an alteration in ParE was also introduced, which probably contributed to ciprofloxacin resistance. The ciprofloxacin-resistant transformants had the donor porB1b sequence, and most of them also had altered serovars. We conclude that alterations in N. gonorrhoeae PorB1b could be involved in ciprofloxacin resistance. |
| Databáze: | OpenAIRE |
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