The Protective Effect of Naringin against Bleomycin-Induced Pulmonary Fibrosis in Wistar Rats

Autor: Sahende Elagoz, Emre Arslanbaş, Köksal Deveci, Nergiz Hacer Turgut, Hüseyin Güngör, Haki Kara
Přispěvatelé: [Turgut, Nergiz H.] Cumhuriyet Univ, Sch Pharm, Dept Pharmacol, TR-58140 Sivas, Turkey -- [Kara, Haki -- Gungor, Huseyin -- Arslanbas, Emre] Cumhuriyet Univ, Sch Vet Med, Dept Pharmacol & Toxicol, TR-58140 Sivas, Turkey -- [Elagoz, Sahende] Cumhuriyet Univ, Sch Med, Dept Pathol, TR-58140 Sivas, Turkey -- [Deveci, Koksal] Cumhuriyet Univ, Sch Med, Dept Biochem, TR-58140 Sivas, Turkey
Rok vydání: 2016
Předmět:
Male
0301 basic medicine
Pulmonary and Respiratory Medicine
medicine.medical_specialty
Article Subject
Pulmonary Fibrosis
Interleukin-1beta
Pharmacology
Bleomycin
Antioxidants
Lipid peroxidation
Random Allocation
03 medical and health sciences
chemistry.chemical_compound
Hydroxyproline
Malondialdehyde
Pulmonary fibrosis
medicine
Animals
Mast Cells
Rats
Wistar
Naringin
lcsh:RC705-779
chemistry.chemical_classification
Glutathione Peroxidase
Antibiotics
Antineoplastic
Dose-Response Relationship
Drug
Superoxide Dismutase
Tumor Necrosis Factor-alpha
business.industry
Glutathione peroxidase
Body Weight
lcsh:Diseases of the respiratory system
General Medicine
medicine.disease
Surgery
Oxidative Stress
Dose–response relationship
030104 developmental biology
chemistry
Flavanones
Lipid Peroxidation
business
Research Article
Zdroj: Pulmonary Medicine
Pulmonary Medicine, Vol 2016 (2016)
ISSN: 2090-1844
2090-1836
DOI: 10.1155/2016/7601393
Popis: WOS: 000377863500001
PubMed ID: 26977316
The aim of the current study was to investigate the protective effect of naringin on bleomycin-induced pulmonary fibrosis in rats. Twenty-four Wistar rats randomly divided into four groups (control, bleomycin alone, bleomycin + naringin 40, and bleomycin + naringin 80) were used. Rats were administered a single dose of bleomycin (5mg/kg; via the tracheal cannula) alone or followed by either naringin 40 mg/kg (orally) or naringin 80mg/kg (orally) or water (1mL, orally) for 14 days. Rats and lung tissue were weighed to determine the lung index. TNF-alpha and IL-1 beta levels, hydroxyproline content, and malondialdehyde (MDA) levels were assayed. Glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities were determined. Tissue sections were stained with hematoxylin-eosin, Masson's trichrome, and 0.1% toluidine blue. TNF-alpha, IL-1 beta, and MDA levels and hydroxyproline content significantly increased (p < 0.01) and GPx and SOD activities significantly decreased in bleomycin group (p < 0.01). Naringin at a dose of 80mg/kg body weight significantly decreased TNF-alpha and IL-1 beta activity, hydroxyproline content, and MDA level (p < 0.01) and increased GPx and SOD activities (p < 0.05). Histological evidence supported the results. These results show that naringin has the potential of reducing the toxic effects of bleomycin and may provide supportive therapy for conventional treatment methods for idiopathic pulmonary fibrosis.
Databáze: OpenAIRE
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