Bat coronaviruses and experimental infection of bats, the Philippines
| Autor: | Phillip A. Alviola, Tetsuya Mizutani, Tsutomu Omatsu, Koichiro Iha, Hiroomi Akashi, Naoya Ueda, Hikaru Fujii, Joseph S. Masangkay, Shuetsu Fukushi, Shumpei Watanabe, Satoshi Taniguchi, Shumpei Tsuda, Noriyo Nagata, Shigeru Morikawa, Yasuhiro Yoshikawa, Maiko Endoh, Shigeru Kyuwa, Kentaro Kato, Yukinobu Tohya |
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| Rok vydání: | 2010 |
| Předmět: |
Microbiology (medical)
animal structures Epidemiology Sequence analysis viruses Philippines Molecular Sequence Data Prevalence bats coronavirus RNA-dependent RNA polymerase lcsh:Medicine severe acute respiratory syndrome Biology medicine.disease_cause Genetic analysis Virus lcsh:Infectious and parasitic diseases Chiroptera Cynopterus brachyotis medicine Animals lcsh:RC109-216 Phylogeny Coronavirus SARS Base Sequence Reverse Transcriptase Polymerase Chain Reaction Research lcsh:R virus diseases food and beverages Sequence Analysis DNA Bat coronavirus the Philippines biology.organism_classification RNA-Dependent RNA Polymerase Virology zoonoses Infectious Diseases Viral replication experimental infection RNA Viral Coronavirus Infections |
| Zdroj: | Emerging Infectious Diseases Emerging Infectious Diseases, Vol 16, Iss 8, Pp 1217-1223 (2010) |
| ISSN: | 1080-6059 |
| Popis: | Virus-infected fruit bats showed no signs of clinical infection. Fifty-two bats captured during July 2008 in the Philippines were tested by reverse transcription–PCR to detect bat coronavirus (CoV) RNA. The overall prevalence of virus RNA was 55.8%. We found 2 groups of sequences that belonged to group 1 (genus Alphacoronavirus) and group 2 (genus Betacoronavirus) CoVs. Phylogenetic analysis of the RNA-dependent RNA polymerase gene showed that groups 1 and 2 CoVs were similar to Bat-CoV/China/A515/2005 (95% nt sequence identity) and Bat-CoV/HKU9–1/China/2007 (83% identity), respectively. To propagate group 2 CoVs obtained from a lesser dog-faced fruit bat (Cynopterus brachyotis), we administered intestine samples orally to Leschenault rousette bats (Rousettus leschenaulti) maintained in our laboratory. After virus replication in the bats was confirmed, an additional passage of the virus was made in Leschenault rousette bats, and bat pathogenesis was investigated. Fruit bats infected with virus did not show clinical signs of infection. |
| Databáze: | OpenAIRE |
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