Comparison between digital and optical microscopy: Analysis in a mouse gut inflammation model
| Autor: | Isabelle Mazza Guimarães, Sylvia M. N. Campos, Airton Pereira e Silva, Gerlinde Agate Platais Brasil Teixeira |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Gut inflammation Pathology medicine.medical_specialty General Neuroscience General Medicine Method of analysis Articles Biology digestive system General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Trustworthiness 030220 oncology & carcinogenesis Whole slide image medicine General Pharmacology Toxicology and Pharmaceutics Virtual microscopy Biomedical engineering |
| Popis: | Virtual microscopy is currently widely used for various purposes, such as teaching, archiving, collaborations and research. Although the cost of this technique has reduced, it continues to be expensive for the majority of laboratories in developing countries. The Graduate Program in Pathology at the Federal Fluminense University (Niteroi, Brazil) has acquired equipment for virtual microscopy. However, this novel method faced prejudice, as students and technicians were skeptical about its reliability. Thus, the aim of the current study was to evaluate whether virtual microscopy is a reliable method of analysis for our research. Thus, a mouse gut inflammation model developed by our research group was used in the present study. Analysis was performed using optical microscopy and digital imaging using the APERIO scanning system and the ImageScope® software. Intestinal epithelial cells (IECs), intra epithelial leucocytes (IEL), and villi number and area were evaluated. No significant differences were observed in villi number, IEC and IEL; however, the villi area was significantly smaller when measured using the computer. Thus, the present study indicates that virtual microscopy is a trustworthy method for research purposes. |
| Databáze: | OpenAIRE |
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