Rapid generation of sequence variation during primary HIV-1 infection
| Autor: | I. S. Y. Chen, S. Pang, David D. Ho, T. Moudgil, E. S. Daar, Y. Shlesinger |
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| Rok vydání: | 1992 |
| Předmět: |
Sexually transmitted disease
Adult Male Immunology Blotting Western Molecular Sequence Data HIV Infections Biology medicine.disease_cause Virus law.invention law Sequence Homology Nucleic Acid Genetic variation medicine Immunology and Allergy Humans Amino Acid Sequence Seroconversion Polymerase chain reaction Genetics Mutation Base Sequence Nucleic acid sequence Genetic Variation Virology Infectious Diseases HIV-1 Viral disease |
| Zdroj: | AIDS (London, England). 6(5) |
| ISSN: | 0269-9370 |
| Popis: | OBJECTIVE HIV-1 undergoes extensive genetic variation in infected individuals. The extent of genetic variation has been examined in patients with AIDS, but little is known regarding the appearance of HIV-1 genetic variation immediately following infection during the primary phase of HIV-1 infection prior to seroconversion. DESIGN We examined HIV-1 genetic variation during this early phase of HIV-1 infection by polymerase chain reaction (PCR) and nucleotide sequence analysis of the V4 by polymerase chain reaction (PCR) and nucleotide sequence analysis of the V4 variable region and the CD4-binding domain. RESULTS Our results demonstrate that extensive sequence variation is seen early after infection, although a predominant HIV-1 species is maintained. CONCLUSIONS The type of variants that occur are dynamic, changing over time, and the mutations seen are consistent with those expected from random occurrence, unlike the pattern of variation previously reported during later stages of disease. |
| Databáze: | OpenAIRE |
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