Polymeric stationary phases based on poly(butylene terephthalate) and poly(4-vinylpirydine) in the analysis of polyphenols using supercritical fluid chromatography. Application to bee pollen
| Autor: | José L. Bernal, Laura Toribio, Ana M. Ares, Sandra Arranz |
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| Rok vydání: | 2018 |
| Předmět: |
Polyesters
01 natural sciences Biochemistry p-Coumaric acid Cinnamic acid Analytical Chemistry chemistry.chemical_compound Limit of Detection Trifluoroacetic acid Pressure Animals Chromatography 010405 organic chemistry Chemistry 010401 analytical chemistry Organic Chemistry Supercritical fluid extraction Temperature Polyphenols Reproducibility of Results Chromatography Supercritical Fluid General Medicine Bees 0104 chemical sciences Polyphenol Bee pollen Supercritical fluid chromatography Pollen Polyvinyls Methanol |
| Zdroj: | Journal of chromatography. A. 1572 |
| ISSN: | 1873-3778 |
| Popis: | Two new polymer-based stationary phases; DCpak PBT (poly(butylene terephthalate)) and DCpak P4VP (poly(4-vinylpirydine)) were evaluated for the analysis of polyphenols using supercritical fluid chromatography (SFC). The compounds studied included phenolic acids and flavonoids. The different variables that influence the chromatographic separation, such as type and percentage of organic modifier, additive, pressure and temperature were examined. Using the DCpak P4VP column the retention was exceptionally high, obtaining better results with the DCpak PBT column. The separation of nine polyphenols was achieved using a gradient of modifier (methanol with 0.1% trifluoroacetic acid) from 5 to 50%, a pressure of 150 bar, a temperature of 35 °C and a flow-rate of 2 mL/min. The use of additives was necessary in order to obtain good peak shapes and efficiencies, achieving the best results with trifluoroacetic acid. LODs and LOQs values were lower than 5 μg/mL in all the cases; meanwhile, the %RSD values for method repeatability and inter-day reproducibility were lower than 3% and 10% respectively. Finally, the proposed method was successfully applied to the analysis of polyphenols in commercial bee pollen; four compounds, namely cinnamic acid, p-coumaric acid, catechin and quercetin were identified and quantified. |
| Databáze: | OpenAIRE |
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