Transcriptome profiling of Diachasmimorpha longicaudata towards useful molecular tools for population management

Autor: Silvia Beatriz Lanzavecchia, Maximo Rivarola, Alejandra Carla Scannapieco, Sergio Alberto González, Marisa Diana Farber, Jorge Luis Cladera, M. Constanza Mannino
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Male
Wasps
Population genetics
Genome
Transcriptome
purl.org/becyt/ford/1 [https]
education.field_of_study
High-Throughput Nucleotide Sequencing
Bioquímica y Biología Molecular
Marcadores Genéticos
Larva
Microsatellite
Female
Functional genomics
Diachasmimorpha longicaudata
CIENCIAS NATURALES Y EXACTAS
Biotechnology
Research Article
Genetic Markers
DIACHASMIMORPHA LONGICAUDATA
GENE EXPRESSION
Expresión Génica
Population
Marcadores Moleculares
Genomics
Locus (genetics)
MOLECULAR MARKERS
Biology
Ciencias Biológicas
03 medical and health sciences
Botany
Genetics
Animals
TRANSCRIPTOMICS
education
Transcriptomics
purl.org/becyt/ford/1.6 [https]
SEX DETERMINATION
Biosteres Longicaudatus
Gene Expression Profiling
fungi
Computational Biology
Genetic Variation
Reproducibility of Results
Molecular markers
Molecular Sequence Annotation
Sex Determination Processes
Sex determination
030104 developmental biology
Gene Ontology
Genetics
Population
Evolutionary biology
Gene expression
Determinación del Sexo
Microsatellite Repeats
Zdroj: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
BMC Genomics
BMC Genomics 17:793 (2016)
INTA Digital (INTA)
Instituto Nacional de Tecnología Agropecuaria
instacron:INTA
Popis: Background: Diachasmimorpha longicaudata (Hymenoptera: Braconidae) is a solitary parasitoid of Tephritidae (Diptera) fruit flies of economic importance currently being mass-reared in bio-factories and successfully used worldwide. A peculiar biological aspect of Hymenoptera is its haplo-diploid life cycle, where females (diploid) develop from fertilized eggs and males (haploid) from unfertilized eggs. Diploid males were described in many species and recently evidenced in D. longicaudata by mean of inbreeding studies. Sex determination in this parasitoid is based on the Complementary Sex Determination (CSD) system, with alleles from at least one locus involved in early steps of this pathway. Since limited information is available about genetics of this parasitoid species, a deeper analysis on D. longicaudata's genomics is required to provide molecular tools for achieving a more cost effective production under artificial rearing conditions. Results: We report here the first transcriptome analysis of male-larvae, adult females and adult males of D. longicaudata using 454-pyrosequencing. A total of 469766 reads were analyzed and 8483 high-quality isotigs were assembled. After functional annotation, a total of 51686 unigenes were produced, from which, 7021 isotigs and 20227 singletons had at least one BLAST hit against the NCBI non-redundant protein database. A preliminary comparison of adult female and male evidenced that 98 transcripts showed differential expression profiles, with at least a 10-fold difference. Among the functionally annotated transcripts we detected four sequences potentially involved in sex determination and three homologues to two known genes involved in the sex determination cascade. Finally, a total of 4674SimpleSequence Repeats (SSRs) were in silico identified and characterized. Conclusion: The information obtained here will significantly contribute to the development of D. longicaudata functional genomics, genetics and population-based genome studies. Thousands of new microsatellite markers were identified as toolkits for population genetics analysis. The transcriptome characterized here is the starting point to elucidate the molecular bases of the sex determination mechanism in this species. Fil: Mannino, Maria Constanza. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rivarola, Maximo Lisandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: González, Sergio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina Fil: Farber, Marisa Diana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Databáze: OpenAIRE
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