nmb, a novel gene, is expressed in low-metastatic human melanoma cell lines and xenografts

Autor: Henri P.J. Bloemers, Dirk J. Ruiter, G.N.P. van Muijen, N. Ajubi, W.G.J. Degen, Marian A. J. Weterman, I.M.R. van Dinter
Přispěvatelé: Other departments
Rok vydání: 1995
Předmět:
Cancer Research
Molecular Sequence Data
Transplantation
Heterologous
Gene Expression
Mice
Nude
Biology
Differential diagnosis of benign versus malignant melanocytic lesions using immunohistochemistry and in situ hybridization
Mice
chemistry.chemical_compound
Neoplasms
Complementary DNA
Gene expression
Tumor Cells
Cultured
Animals
Humans
Genes
Tumor Suppressor
De rol van chromosoomafwijkingen en (anti-)oncogenen in humane tumoren
Amino Acid Sequence
Cloning
Molecular
GeneralLiterature_REFERENCE(e.g.
dictionaries
encyclopedias
glossaries)
Melanoma
Gene
Genomic Library
GPNMB
Base Sequence
Sequence Homology
Amino Acid
cDNA library
fungi
DNA
Neoplasm
Transfection
Molecular biology
Oncology
chemistry
Cell culture
The role of chromosomal aberrations and (anti-)oncogenes in human tumours
Differentiële diagnose tussen goed- en kwaadaardige melanocytaire afwijkingen met behulp van immunohistochemie en in situ hybridisatie
Glembatumumab vedotin
Neoplasm Transplantation
Zdroj: International journal of cancer. Journal international du cancer, 60(1), 73-81. Wiley-Liss Inc.
International Journal of Cancer, 60, pp. 73-81
International Journal of Cancer, 60, 73-81
International Journal of Cancer, 60, 1, pp. 73-81
ISSN: 1097-0215
0020-7136
DOI: 10.1002/ijc.2910600111
Popis: From a subtractive cDNA library, we isolated several cDNA clones which showed differential expression between highly and lowly metastatic human melanoma cell lines. One clone, designated nmb, showed preferential expression in the low-meta-static cell lines and was chosen for further characterization. Sequence analysis revealed that this clone represents a novel gene, encoding a putative transmembrane glycoprotein which showed the highest homology to the precursor of pMEL17, a melanocyte-specific protein. nmb RNA expression was absent in most tumor-cell lines tested and not restricted to the melanocytic lineage. Transfection of a partial nmb cDNA into a highly metastatic melanoma cell line (BLM) resulted, in 2 of 3 transfectants, in slower subcutaneous tumor growth and, in 1 of 3 transfectants, in reduction of the potential for spontaneous metastasis in nude mice. © 1995 Wiley-Liss, Inc.
Databáze: OpenAIRE
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