Early detection of E. coli and total coliform using an automated, colorimetric and fluorometric fiber optics-based device
| Autor: | Aydogan Ozcan, Can Firat Usanmaz, Kevin de Haan, Sabiha Tok, Derek Tseng, Hatice Ceylan Koydemir |
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| Přispěvatelé: | Usanmaz, Can Fırat, Özcan, Aydoğan |
| Rok vydání: | 2019 |
| Předmět: |
Optical fiber
Biomedical Engineering Early detection Bioengineering 02 engineering and technology 01 natural sciences Biochemistry law.invention Raspberry pi Automation Fluorescent light law Escherichia coli Fluorometry Optical Fibers Blue light Chromatography 010401 analytical chemistry General Chemistry 021001 nanoscience & nanotechnology Water sample 0104 chemical sciences Total coliform Contaminated water Environmental science Colorimetry 0210 nano-technology |
| Zdroj: | Lab on a Chip |
| ISSN: | 1473-0189 |
| Popis: | Lack of access to clean water is a major global issue that affects millions of people worldwide. Drinking contaminated water can be extremely hazardous, so it is imperative that it is tested sufficiently. One method commonly used to determine the quality of water is testing for both E. coli and total coliform. Here, we present a cost-effective and automated device which can concurrently test drinking water samples for both E. coli and total coliform using an EPA-approved reagent. Equipped with a Raspberry Pi microcontroller and camera, we perform automated periodic measurements of both the absorption and fluorescence of the water under test over 24 hours. In each test, 100 mL of the water sample is split into a custom designed 40-well plate, where the transmitted blue light and the fluorescent light (under UV excitation) are collected by 520 individual optical fibers. Images of these fiber outputs are then acquired periodically, and digitally processed to determine the presence of the bacteria in each well of the 40-well plate. We demonstrate that this cost-effective device, weighing 1.66 kg, can automatically detect the presence of both E. coli and total coliform in drinking water within ∼16 hours, down to a level of one colony-forming unit (CFU) per 100 mL. Furthermore, due to its automated analysis, this approach is also more sensitive than a manual count performed by an expert, reducing the time needed to determine whether the water under test is safe to drink or not. |
| Databáze: | OpenAIRE |
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