Light-emitting diode irradiation using 660 nm promotes human fibroblast HSP90 expression and changes cellular activity and morphology

Autor: So-Young Chang, Sun-Hyang Choi, Jin Chul Ahn, SangJoon Mo, Phil-Sang Chung, Raktim Biswas, Min Young Lee
Rok vydání: 2019
Předmět:
Cell Survival
General Physics and Astronomy
01 natural sciences
General Biochemistry
Genetics and Molecular Biology
010309 optics
Mitochondrial Proteins
chemistry.chemical_compound
Adenosine Triphosphate
Hsp27
Heat shock protein
0103 physical sciences
medicine
Fluorescence microscope
Humans
General Materials Science
HSP70 Heat-Shock Proteins
Viability assay
HSP90 Heat-Shock Proteins
Low-Level Light Therapy
skin and connective tissue diseases
Fibroblast
Cell Proliferation
Skin
Wound Healing
biology
Chemistry
Gene Expression Profiling
010401 analytical chemistry
General Engineering
General Chemistry
Chaperonin 60
Fibroblasts
Hsp90
Immunohistochemistry
0104 chemical sciences
Cell biology
Blot
medicine.anatomical_structure
Gene Expression Regulation
Microscopy
Fluorescence
biology.protein
sense organs
Adenosine triphosphate
Zdroj: Journal of biophotonicsREFERENCES. 12(9)
ISSN: 1864-0648
Popis: We evaluated changes in cell viability and morphology in response to low-level light irradiation and underlying variations in the levels of heat shock proteins (HSPs). Human fibroblasts were irradiated with a light-emitting diode (LED) array at 660 nm (50 mW for 15, 30, and 60 minutes). Cell viability and morphological changes were evaluated via epifluorescence analysis; we also assessed cell viability and length changes. The expression levels of adenosine triphosphate (ATP) and various HSPs (HSP27, 60, 70, and 90) were analyzed by immunohistochemical staining, Western blotting and microarray analysis. After LED irradiation, cellular viability and morphology changed. Of the several HSPs analyzed, the HSP90 level increased significantly, suggesting that this protein played roles in the morphological and cellular changes. Thus, low-level irradiation triggered cellular changes mediated by increased HSP90 expression; this may explain why skin irradiation enhances wound-healing.
Databáze: OpenAIRE
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