MTHFR gene polymorphisms in hypothyroidism and hyperthyroidism among Jordanian females

Autor: Omar Alqaisi, Tamer Altamimi, Nadera A. Altork, Abdullah Alhouri, Diala W. Abu-Hassan, Baha Mustafa, Zakaria W. Shkoukani
Rok vydání: 2019
Předmět:
Adult
medicine.medical_specialty
Genotype
endocrine system diseases
Endocrinology
Diabetes and Metabolism
lcsh:Medicine
lcsh:Diseases of the endocrine glands. Clinical endocrinology
Hyperthyroidism
Polymerase Chain Reaction
Polymorphism
Single Nucleotide
Gastroenterology
thyroid
folic acid
Young Adult
Hypothyroidism
Risk Factors
Internal medicine
medicine
Humans
Genetic Predisposition to Disease
Alleles
Methylenetetrahydrofolate Reductase (NADPH2)
thyroxine
lcsh:RC648-665
Jordan
biology
business.industry
Thyroid disease
lcsh:R
Haplotype
Thyroid
Case-control study
deficiency
DNA Methylation
Middle Aged
medicine.disease
Genotype frequency
medicine.anatomical_structure
Haplotypes
Case-Control Studies
Methylenetetrahydrofolate reductase
biology.protein
Female
Restriction fragment length polymorphism
business
metabolism
Polymorphism
Restriction Fragment Length
Zdroj: Archives of Endocrinology and Metabolism, Iss 0 (2019)
Archives of Endocrinology and Metabolism, Issue: ahead, Published: 02 MAY 2019
Archives of Endocrinology and Metabolism v.63 n.3 2019
Arquivos de Endocrinologia e Metabolismo
Sociedade Brasileira de Endocrinologia e Metabologia (SBEM)
instacron:SBEM
Archives of Endocrinology and Metabolism, Volume: 63, Issue: 3, Pages: 280-287, Published: 02 MAY 2019
ISSN: 2359-4292
2359-3997
DOI: 10.20945/2359-3997000000133
Popis: Objective Methylenetetrahydrofolate reductase (MTHFR) is involved in DNA methylation that is associated with autoimmune pathology. We investigated the association between MTHFR genetic polymorphisms at g.677C>T and g.1298A>C and their haplotypes, and the risk of thyroid dysfunction among Jordanian females. Subjects and methods A case-control study involving 98 hypothyroidism cases, 66 hyperthyroidism cases and 100 controls was conducted. Polymerase chain reaction/restriction fragment length polymorphism technique was performed to determine genotypes. Statistical analysis using SPSS software was performed. Results Genetic analysis showed a significant difference in genotype frequency of g.1298A>C between cases, and controls [hypothyroidism: AA (45.9%), AC (37.8%), CC (16.3%); hyperthyroidism: AA (9.1%), AC (69.7%), CC (21.2%); controls: AA (37.8%), AC (29.6%), CC (32.7%); CChypo vs. AAhypo: 2.55, 95% CI: (1.18-5.52); OR at least on Chypo: 1.79, 95% CI: (1.07-2.99)]; CChyper vs. AAhyper: 4.01, 95% CI: (1.79-9.01); OR at least on Chyper: 0.18, 95% CI: (0.07-0.48)]. There was no significant difference in genotype frequency of g.677C>T between cases and controls [hypothyroidism: CC (50.0%), CT (32.7%), TT (17.3%); hyperthyroidism: CC (77.3%), CT (15.2%), TT (7.6%); controls: CC (55.6%), CT (32.3%), TT (12.1%)]. There was a significant difference of MTHFR haplotypes among hypothyroidism cases and controls. TA and CC had a lower hypothyroidism risk whereas; TC showed a higher risk. Conclusions g.1298A>C genetic polymorphism of MTHFR may modulate the risk of thyroid disease. CC, TA, and TC haplotypes affect the risk of hypothyroidism. Larger samples should be included in the future to verify the role of MTHFR polymorphisms in thyroid diseases.
Databáze: OpenAIRE
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