Use of Low-Dose Irradiation To Evaluate the Radiation Sensitivity of Escherichia coli O157:H7, Non-O157 Verotoxigenic Escherichia coli, and Salmonella in Phosphate-Buffered Saline
| Autor: | Richard A. Holley, Alexander Gill, Devapriya Kundu |
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| Rok vydání: | 2013 |
| Předmět: |
Serotype
Salmonella Food Safety Shiga-Toxigenic Escherichia coli X-Rays Phosphate buffered saline Dose-Response Relationship Radiation Low dose irradiation Biology Escherichia coli O157 medicine.disease_cause Radiation Tolerance Microbiology fluids and secretions Radiation sensitivity VTEC Food Irradiation Verotoxigenic Escherichia coli medicine Humans Escherichia coli Food Science |
| Zdroj: | Journal of Food Protection. 76:1438-1442 |
| ISSN: | 0362-028X |
| DOI: | 10.4315/0362-028x.jfp-12-492 |
| Popis: | Verotoxigenic Escherichia coli (VTEC) and Salmonella are major foodborne pathogens, but very little information is available on the radiation resistance of a sufficiently diverse group of these pathogens. The objective of this study was to evaluate the sensitivity of E. coli O157:H7, non-O157 VTEC, and Salmonella to a low-dose ionizing radiation treatment. Test organisms were 6 serovars of Salmonella, 5 strains of E. coli O157:H7, and 27 strains of non-O157 VTEC (representing 19 serotypes). Decimal reduction doses (D-values) for individual strains were determined in phosphate-buffered saline using an X-ray source. The viability of the bacterial cells declined with an increase in absorbed dose from 0 to 0.3 kGy. The more resistant test strains were screened at 0.5 and 0.7 kGy. All six Salmonella strains survived at 0.5 and 0.7 kGy; however, only 11 VTEC survived at 0.7 kGy. After the 0.3-kGy treatment, both E. coli O157:H7 and non-O157 VTEC had D-values with similar means and ranges (0.028 to 0.123 and 0.037 to 0.127 kGy, respectively), with no significant differences (P0.05). Salmonella strains had a slightly higher range of D-values (0.061 to 0.147 kGy) and a mean D-value that was significantly higher (P0.05) than that of both the E. coli O157:H7 and non-O157 VTEC groups. |
| Databáze: | OpenAIRE |
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