Fluoride exposure cause colon microbiota dysbiosis by destroyed microenvironment and disturbed antimicrobial peptides expression in colon
| Autor: | Wen-peng Zhao, Hong-wei Wang, Shi-quan Zhu, Jing Liu, Jing Zhao, Bo Han, Bian-hua Zhou |
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| Rok vydání: | 2021 |
| Předmět: |
Pore Forming Cytotoxic Proteins
Colon Health Toxicology and Mutagenesis Antimicrobial peptides Gut flora Toxicology Immunofluorescence Microbiology chemistry.chemical_compound Fluorides Mice RNA Ribosomal 16S medicine Animals chemistry.chemical_classification medicine.diagnostic_test biology General Medicine medicine.disease biology.organism_classification Pollution Gastrointestinal Microbiome chemistry 16s rrna gene sequencing Biomarker (medicine) Dysbiosis Female Glycoprotein Fluoride |
| Zdroj: | Environmental pollution (Barking, Essex : 1987). 292 |
| ISSN: | 1873-6424 |
| Popis: | Colon microenvironment and microbiota dysbiosis are closely related to various human metabolic diseases. In this study, a total of 72 healthy female mice were exposed to fluoride (F) (0, 25, 50 and 100 mg/L F−) in drinking water for 70 days. The effect of F on intestinal barrier and the diversity and composition in colon microbiota have been evaluated. Meanwhile, the relationship among F-induced colon microbiota alterations and antimicrobial peptides (AMPs) expression and short-chain fatty acids (SCFAs) level also been assessed. The results suggested that F decreased the goblet cells number and glycoprotein expression in colon. And further high-throughput 16S rRNA gene sequencing result demonstrated that F exposure induced the diversity and community composition of colonic microbiota significantly changes. Linear Discriminant Analysis Effect Size (LEfSe) analysis identified 11 predominantly characteristic taxa which may be the biomarker in response to F exposure. F-induced intestinal microbiota perturbations lead to the significantly decreased SCFAs levels in colon. Immunofluorescence results showed that F increased the protein expression of interleukin-17A (IL-17A) and IL-22 (P |
| Databáze: | OpenAIRE |
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