Modification of anin vitromodel simulating the whole digestive process to investigate cellular endpoints of chemoprevention
| Autor: | Ursula Obst, Michael Glei, Kerstin Scheu, Jürgen Hollmann, Gerald Brenner-Weiss, Katrin Stein, Anke Borowicki, Norbert Wachter, Daniel Scharlau, Meinolf Georg Lindhauer |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Colon
Flour Medicine (miscellaneous) Adenocarcinoma Biology Gut flora Models Biological Cell Line Ox gall chemistry.chemical_compound Cell Line Tumor Animals Bile Humans Cytotoxic T cell Triticum Carcinogen Nutrition and Dietetics Deoxycholic acid Cholic acid food and beverages Cholic Acids Fatty Acids Volatile biology.organism_classification In vitro chemistry Biochemistry Colonic Neoplasms Fermentation Cattle Digestion Edible Grain |
| Zdroj: | British Journal of Nutrition. 105:678-687 |
| ISSN: | 1475-2662 0007-1145 |
| DOI: | 10.1017/s0007114510004320 |
| Popis: | In vitrogut fermentation systems are relevant tools to study health benefits of foodstuffs. Most of them are commonly used to investigate the degradation of nutrients or the development of gut flora. Using these models, strong cytotoxic effects of the resulting samples on cultured cells were observed. Hence, the aim of the present study was to develop a modifiedin vitrofermentation model that simulates the whole digestive tract and generates fermented samples that are suitable for testing in cell culture experiments. Wholemeal wheat flour (wwf) was digested and fermentedin vitrowith a fermentation model using different ox gall concentrations (41·6 and 0·6 g/l). The resulting fermentation supernatants (fs) were characterised for metabolites and biological effects in HT29 cells. The fermentation of wwf increased chemopreventive SCFA and decreased carcinogenic deoxycholic acid (DCA). The strong cytotoxic effects of the fs, which were partly due to cholic acid and DCA, were diminished by lowering the ox gall concentration, allowing the use of the samples in cell culture experiments. In conclusion, anin vitrodigestion model, which can be used to study the effects of foodstuffs on chemoprevention and gut health in colon cells, is introduced and its physiological relevance is demonstrated. |
| Databáze: | OpenAIRE |
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