QN1/KIAA1009: a new essential protein for chromosome segregation and mitotic spindle assembly
| Autor: | Patricia Crisanti, C Klein, A. Leon, A Gely, B. Omri |
|---|---|
| Přispěvatelé: | Physiopathologie des Maladies Oculaires : Innovations Therapeutiques, Université Pierre et Marie Curie - Paris 6 (UPMC)-IFR58-Institut National de la Santé et de la Recherche Médicale (INSERM), Régulations et communications cellulaires (RCC), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Diderot - Paris 7 (UPD7)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Pierre et Marie Curie - Paris 6 (UPMC), Crisanti Lassiaz, Patricia, Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
MESH: Cell Death
Cancer Research MESH: RNA Sma Polo-like kinase MESH: Base Sequence Spindle pole body Chromosome segregation 0302 clinical medicine MESH: Eye Proteins Chromosome Segregation Tumor Cells Cultured MESH: Animals RNA Small Interfering [SDV.BDD]Life Sciences [q-bio]/Development Biology Adenosine Triphosphatases 0303 health sciences Cell Death Quail Cell biology 030220 oncology & carcinogenesis Female [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] Centrosome separation Microtubule-Associated Proteins Molecular Sequence Data MESH: Chromosome Segregation Mitosis Breast Neoplasms Pheochromocytoma Spindle Apparatus [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology 03 medical and health sciences biology.animal [SDV.BDD] Life Sciences [q-bio]/Development Biology Genetics MESH: Adenosine Triphosphatases Animals Humans MESH: Pheochromocytoma [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] Eye Proteins Molecular Biology [SDV.BC] Life Sciences [q-bio]/Cellular Biology 030304 developmental biology MESH: Mitotic Spindle Apparatus MESH: Molecular Sequence Data MESH: Humans Base Sequence MESH: Mitosis Molecular biology Spindle apparatus Rats MESH: Microtubule-Associated Proteins Centrosome MESH: Female MESH: Breast Neoplasms |
| Zdroj: | Oncogene Oncogene, Nature Publishing Group, 2006, 25 (13), pp.1887-95. ⟨10.1038/sj.onc.1209215⟩ Oncogene, 2006, 25 (13), pp.1887-95. ⟨10.1038/sj.onc.1209215⟩ |
| ISSN: | 0950-9232 1476-5594 |
| DOI: | 10.1038/sj.onc.1209215⟩ |
| Popis: | We previously reported the involvement of QN1 (quail neuroretina 1) protein in cell cycle control during retinal development. We show here that QN1 is an ATPase conserved through evolution, from fugu to humans. We show that chicken/quail QN1 protein is orthologous to the KIAA1009 protein in humans, the function of which was not known. We demonstrate here for the first time that QN1/KIAA1009 protein is located at the spindle poles of the mitotic apparatus and at centrosomes during mitosis. The siRNA-mediated depletion of KIAA1009 led to abnormal mitosis with chromosome segregation defects and abnormal centrosome separation leading to the death of PC12 and MCF7 cells. Thus, QN1/KIAA1009 is a new microtubule-associated ATPase involved in cell division. |
| Databáze: | OpenAIRE |
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