Antifungal Activity Directed Toward the Cell Wall by 2-Cyclohexylidenhydrazo- 4-Phenyl-Thiazole Against Candida albicans
| Autor: | Cleudiomar Inácio Lino, Susana Johann, Ana Paula Possa, Carlos A. Rosa, Beth Burgwyn Fuchs, Nayara Cristina Fonseca, Renata Barbosa de Oliveira, Jaqueline Maria Siqueira Ferreira, Nívea Pereira de Sá, Pilar Pérez, Eleftherios Mylonakis, Lana Ivone Barreto Cruz, Beatriz M. Borelli |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microbiology (medical) endocrine system Antifungal Agents endocrine system diseases 030106 microbiology Mutant β-glucan Microbial Sensitivity Tests Microbiology Cell wall 03 medical and health sciences Minimum inhibitory concentration 2-cyclohexylidenhydrazo-4-phenyl-thiazole Cell Wall Candida albicans Animals Humans heterocyclic compounds Caenorhabditis elegans neoplasms Glucan Pharmacology chemistry.chemical_classification Virulence biology Chemistry Candidiasis Epithelial Cells Hep G2 Cells General Medicine Haemolysis biology.organism_classification digestive system diseases Corpus albicans Thiazoles 030104 developmental biology Toxicity Molecular Medicine Anticandidal activity Synthetic compounds |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1871-5265 |
| DOI: | 10.2174/1871526518666180531101605 |
| Popis: | [Background] The increasing incidence of invasive forms of candidiasis and resistance to antifungal therapy leads us to seek new and more effective antifungal compounds. Objectives: To investigate the antifungal activity and toxicity as well as to evaluate the po-tential targets of 2-cyclohexylidenhydrazo-4-phenyl-thiazole (CPT) in Candida albicans. [Methods] The antifungal activity of CPT against the survival of C. albicans was inves-tigated in Caenorhabditis elegans. Additionally, we determined the effect of CPT on the inhibition of C. albicans adhesion capacity to buccal epithelial cells (BECs), the toxicity of CPT in mammalian cells, and the potential targets of CPT in C. albicans. [Results] CPT exhibited a minimum inhibitory concentration (MIC) value of 0.4-1.9 μg/mL. Furthermore, CPT at high concentrations (>60 x MIC) showed no or low toxici-ty in HepG2 cells and [Conclusion] In conclusion, CPT appears to target the cell wall of C. albicans, exhibits low toxicity in mammalian cells, and prolongs the survival of C. elegans infected with C. albicans. |
| Databáze: | OpenAIRE |
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