Fabrication of a Neotrachea Using Engineered Cartilage
| Autor: | Mark Weidenbecher, James E. Dennis, Harvey M. Tucker, Amad Awadallah |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Biocompatibility Cell Culture Techniques Silicones Article Epithelium Surgical Flaps Tissue Culture Techniques Chondrocytes Ear Cartilage Tissue engineering Restenosis Cartilage transplantation medicine Animals Pliability Abdominal Muscles Tissue Engineering business.industry Cartilage Skin Transplantation medicine.disease Elasticity Biomechanical Phenomena Surgery Tracheal Stenosis Trachea medicine.anatomical_structure Otorhinolaryngology Feasibility Studies Stents Rabbits Stress Mechanical business |
| Zdroj: | The Laryngoscope. 118:593-598 |
| ISSN: | 1531-4995 0023-852X |
| DOI: | 10.1097/mlg.0b013e318161f9f8 |
| Popis: | Objectives: Surgical management of long-segment tracheal stenosis is an ongoing problem. Many types of tracheal prostheses have been tried but with limited success because of immune rejection, graft ischemia, or restenosis. Tissue engineered cartilage may offer a solution to this problem, although scaffolds, which are currently often used for support, can lead to biocompatibility problems. This study investigated the feasibility of scaffold-free cartilage to tissue engineer a vascularized neotrachea in rabbits. Study Design: Animal study. Methods: Autologous neotracheal constructs were implanted in the abdomen of six New Zealand white rabbits. Auricular chondrocytes were used to engineer scaffold-free cartilage sheets. A muscle flap raised from the external abdominal oblique muscle and the engineered cartilage were wrapped around a silicone stent to fabricate a vascularized neotrachea in vivo. In two of the six rabbits, a full thickness skin graft was used to create an epithelial lining. The constructs were harvested after either 6 or 10 weeks. Results: All neotracheal constructs were healthy with well-vascularized and integrated layers. The implanted engineered cartilage underwent a remodeling process, forming a solid tracheal framework. Constructs harvested after 10 weeks proved to have significantly better mechanical properties than after 6 weeks and were comparable with the rabbit's native trachea. Conclusion: Scaffold-free engineered cartilage can successfully fabricate a well-vascularized, autologous neotrachea with excellent mechanical properties. The results suggest that this approach can be used to reconstruct tracheal defects in rabbits. |
| Databáze: | OpenAIRE |
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