Exposure of mammalian cells to 60-Hz magnetic or electric fields: analysis of DNA repair of induced, single-strand breaks
| Autor: | M. E. Frazier, R. F. Jostes, Douglas L. Miller, J. A. Reese, James E. Morris |
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| Rok vydání: | 1990 |
| Předmět: |
Lysis
DNA Repair Physiology DNA repair Somatic cell DNA damage Biophysics DNA Single-Stranded General Medicine In Vitro Techniques Molecular biology chemistry.chemical_compound Nuclear magnetic resonance Electromagnetic Fields chemistry Electric field Humans Radiology Nuclear Medicine and imaging Lymphocytes Cobalt Radioisotopes Isotopes of cobalt DNA Single strand DNA Damage |
| Zdroj: | Bioelectromagnetics. 11(3) |
| ISSN: | 0197-8462 |
| Popis: | DNA damage was induced in isolated human peripheral lymphocytes by exposure at 5 Gy to 60Co radiation. Cells were permitted to repair the DNA damage while exposed to 60-Hz fields or while sham-exposed. Exposed cells were subjected to magnetic (B) or electric (E) fields, alone or in combination, throughout their allotted repair time. Repair was stopped at specific times, and the cells were immediately lysed and then analyzed for the presence of DNA single-strand breaks (SSB) by the alkaline-elution technique. Fifty to 75 percent of the induced SSB were repaired 20 min after exposure, and most of the remaining damage was repaired after 180 min. Cells were exposed to a 60-Hz ac B field of 1 mT; an E field of 1 or 20 V/m; or combined E and B fields of 0.2 V/m and 0.05 mT, 6 V/m and 0.6 mT, or 20 V/m and 1 mT. None of the exposures was observed to affect significantly the repair of DNA SSB. |
| Databáze: | OpenAIRE |
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