Nuclear membranes control symbiotic calcium signaling of legumes
| Autor: | Marisa S. Otegui, Ward Capoen, Giles E. D. Oldroyd, Jongho Sun, Jean-Michel Ané, J. Allan Downie, Muthusubramanian Venkateshwaran, Richard J. Morris, Derin Wysham, Sibylle Hirsch, Hiroki Miwa |
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| Rok vydání: | 2011 |
| Předmět: |
0106 biological sciences
Lipopolysaccharides Nuclear Envelope Molecular Sequence Data chemistry.chemical_element Calcium Biology Endoplasmic Reticulum 01 natural sciences Models Biological Plant Roots Calcium in biology Ion Channels Plant Epidermis Sarcoplasmic Reticulum Calcium-Transporting ATPases Diffusion 03 medical and health sciences Cytosol Medicago truncatula Inner membrane Calcium Signaling Gene Silencing Symbiosis 030304 developmental biology Calcium signaling Plant Proteins 0303 health sciences Multidisciplinary Nucleoplasm Voltage-dependent calcium channel Biological Sciences Calcium ATPase Protein Transport chemistry Biochemistry Cytoplasm Biophysics 010606 plant biology & botany |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 108(34) |
| ISSN: | 1091-6490 |
| Popis: | Nuclear-associated oscillations in calcium act as a secondary messenger in the symbiotic signaling pathway of legumes. These are decoded by a nuclear-localized calcium and calmodulin-dependent protein kinase, the activation of which is sufficient to drive downstream responses. This implies that the calcium oscillations within the nucleus are the predominant signals for legume symbiosis. However, the mechanisms that allow targeted release of calcium in the nuclear region have not been defined. Here we show that symbiosis-induced calcium changes occur in both the nucleoplasm and the perinuclear cytoplasm and seem to originate from the nuclear membranes. Reaction diffusion simulations suggest that spike generation within the nucleoplasm is not possible through transmission of a calcium wave from the cytoplasm alone and that calcium is likely to be released across the inner nuclear membrane to allow nuclear calcium changes. In agreement with this, we found that the cation channel DMI1, which is essential for symbiotic calcium oscillations, is preferentially located on the inner nuclear membrane, implying an essential function for the inner nuclear membrane in symbiotic calcium signaling. Furthermore, a sarco/endoplasmic reticulum calcium ATPase (SERCA) essential for symbiotic calcium oscillations is targeted to the inner nuclear membrane, as well as the outer nuclear membrane and endoplasmic reticulum (ER). We propose that release of calcium across the inner nuclear membrane allows targeted release of the ER calcium store, and efficient reloading of this calcium store necessitates the capture of calcium from the nucleoplasm and nuclear-associated cytoplasm. |
| Databáze: | OpenAIRE |
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