Sequence analysis of the 5′-untranslated region of the human H1 histamine receptor-encoding gene
| Autor: | Badrul A. Chowdhury, Claire M. Fraser, Steven I. Max |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Five prime untranslated region
Sequence analysis Molecular Sequence Data Biology Polymerase Chain Reaction law.invention Receptors Glucocorticoid law Genetics Humans Receptors Histamine H1 Gene Polymerase chain reaction Binding Sites Base Sequence Inverse polymerase chain reaction Sequence Analysis DNA General Medicine TATA Box Molecular biology Blotting Southern genomic DNA Protein Biosynthesis Recombinant DNA Primer (molecular biology) |
| Zdroj: | Gene. 171:309-310 |
| ISSN: | 0378-1119 |
| Popis: | A clone containing the H 1 histamine receptor (H 1 HR)-encoding gene was isolated from a human genomic DNA library. The 5′-UTR of the H 1 HR gene reported here differs upstream from bp −142 from that reported previously [Fukui et al., Biochem. Biophys. Res. Comm. 201 (1994) 894–901]. PCR amplification utilizing primer pairs derived from the 5′-UTR reported herein amplified a DNA fragment of the expected size from human genomic DNA whereas 5′-UTR primers derived from the Fukui et al. sequence did not yield a PCR product. The 5′-UTR of H 1 HR contains potential TATA and CCAAT boxes, a CACCC sequence, potential GREs and other DNA-binding motifs. |
| Databáze: | OpenAIRE |
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