The potential renal toxicity of silver nanoparticles after repeated oral exposure and its underlying mechanisms
| Autor: | Mohammad Javad Assari, Massoud Saidijam, Zahra Gholami Mahmoudian, Zohreh Alizadeh, Nooshin Shabab, Hamed Nosrati, Maryam Sohrabi, Manijeh Hamzepoor |
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| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Brush border Gene Expression Metal Nanoparticles Apoptosis 02 engineering and technology Kidney Blood Urea Nitrogen Andrology 03 medical and health sciences Transforming Growth Factor beta In vivo Internal medicine In Situ Nick-End Labeling Animals Medicine RNA Messenger Rats Wistar Extracellular Matrix Proteins TUNEL assay Epidermal Growth Factor Toxicity Caspase 3 Tumor Necrosis Factor-alpha business.industry Research Body Weight Organ Size 021001 nanoscience & nanotechnology Immunohistochemistry Diseases of the genitourinary system. Urology Staining 030104 developmental biology medicine.anatomical_structure Nephrology Creatinine RC870-923 Chemical and Drug Induced Liver Injury Silver nanoparticles 0210 nano-technology business |
| Zdroj: | BMC Nephrology, Vol 22, Iss 1, Pp 1-12 (2021) BMC Nephrology |
| ISSN: | 1471-2369 |
| Popis: | Background Silver nanoparticles (AgNPs) can accumulate in various organs after oral exposure. The main objective of the current study is to evaluate the renal toxicity induced by AgNPs after repeated oral exposure and to determine the relevant molecular mechanisms. Methods In this study, 40 male Wistar rats were treated with solutions containing 30, 125, 300, and 700 mg/kg of AgNPs. After 28 days of exposure, histopathological changes were assessed using hematoxylin-eosin (H&E), Masson’s trichrome, and periodic acid-Schiff (PAS) staining. Apoptosis was quantified by TUNEL and immunohistochemistry of caspase-3, and the level of expression of the mRNAs of growth factors was determined using RT-PCR. Results Histopathologic examination revealed degenerative changes in the glomeruli, loss of tubular architecture, loss of brush border, and interrupted tubular basal laminae. These changes were more noticeable in groups treated with 30 and 125 mg/kg. The collagen intensity increased in the group treated with 30 mg/kg in both the cortex and the medulla. Apoptosis was much more evident in middle-dose groups (i.e., 125 and 300 mg/kg). The results of RT-PCR indicated that Bcl-2 and Bax mRNAs upregulated in the treated groups (p Conclusion Our observations showed that AgNPs played a critical role in in vivo renal toxicity. |
| Databáze: | OpenAIRE |
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