Studying antibiotic–membrane interactions via X-ray diffraction and fluorescence microscopy
| Autor: | Cheng-Hao Lin, Ming-Tao Lee, Ping-Yuan Huang, Kuan-Rong Lee, Yi-Ting Sun |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
QH301-705.5
medicine.drug_class Antibiotics Nanotechnology General Biochemistry Genetics and Molecular Biology A. baumannii Acinetobacter baumannii Research article medicine Fluorescence microscope polycyclic compounds Lamellar structure Biology (General) GUV LXD lamellar X-ray diffraction Aqueous solution Chemistry Vesicle Sulbactam LXD Penicillin Membrane Cholesterol GUV giant unilamellar vesicle Biophysics medicine.drug |
| Zdroj: | FEBS Open Bio FEBS Open Bio, Vol 5, Iss 1, Pp 515-521 (2015) |
| ISSN: | 2211-5463 |
| Popis: | Highlights • We present antibiotic-induced membrane thinning of a multi-lamellar thin film sample. • Both penicillin and sulbactam are found positioned outside the model membrane in an aqueous solution. • We demonstrate a hybrid method to study the antibiotic–membrane interaction. Antibiotic drug resistance is a serious issue for the treatment of bacterial infection. Understanding the resistance to antibiotics is a key issue for developing new drugs. We used penicillin and sulbactam as model antibiotics to study their interaction with model membranes. Cholesterol was used to target the membrane for comparison with the well-known insertion model. Lamellar X-ray diffraction (LXD) was used to determine membrane thickness using successive drug-to-lipid molar ratios. The aspiration method for a single giant unilamellar vesicle (GUV) was used to monitor the kinetic binding process of antibiotic–membrane interactions in an aqueous solution. Both penicillin and sulbactam are found positioned outside the model membrane, while cholesterol inserts perpendicularly into the hydrophobic region of the membrane in aqueous solution. This result provides structural insights for understanding the antibiotic–membrane interaction and the mechanism of antibiotics. |
| Databáze: | OpenAIRE |
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