An Immunoluminometric Assay for Cardiotrophin-1: A Newly Identified Cytokine Is Present in Normal Human Plasma and Is Increased in Heart Failure
| Autor: | P. F. Downie, J.D. Davies, David B. Barnett, Leong L. Ng, S Talwar, Iain B. Squire |
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| Rok vydání: | 1999 |
| Předmět: |
Male
Cardiotrophin 1 medicine.medical_treatment Molecular Sequence Data Cardiac Output Low Biophysics Peptide Biology Biochemistry Reference Values medicine Animals Humans Amino Acid Sequence Molecular Biology Peptide sequence Immunosorbent Techniques Aged Aged 80 and over Immunoassay chemistry.chemical_classification medicine.diagnostic_test Myocardium Cell Biology Middle Aged Glycoprotein 130 medicine.disease Molecular biology Rats Amino acid Molecular Weight Cytokine chemistry Heart failure Luminescent Measurements Cytokines Electrophoresis Polyacrylamide Gel Female |
| Zdroj: | Biochemical and Biophysical Research Communications. 261:567-571 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1999.1084 |
| Popis: | Cardiotrophin-1, a member of the interleukin-6 related cytokine family which acts via the glycoprotein 130 signalling pathway, may be involved in the process of ventricular remodelling. Its presence in human plasma has never been reported. We have devised a non-radioactive immunoluminometric sensitive and specific assay for CT-1 based on a competitive ligand binding principle. The chemiluminescent label 4-(2-succinimidyl-oxycarbonylethyl)phenyl-10-methylacridinium 9-carboxylate fluorosulfonate was used to label a peptide representing a domain in the middle section of CT-1. Assay of this domain of CT-1 (amino acids 105-120) in patients with heart failure revealed elevated CT-1 values median 87 [range 74.3-182.8] fmol/ml) compared to normal controls (CT-1 median 29.55 [range 6.9-48.3] fmol/ml, P0.0005). The molecular weight of human CT-1 was estimated to be 26.7 kD from sodium dodecyl sulphate polyacrylamide gel electrophoresis. This is the first quantitative assessment of CT-1 in humans. Furthermore, this is the first demonstration of significant elevation of plasma CT-1 in patients with heart failure. |
| Databáze: | OpenAIRE |
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