Broomrape infestation in carrot (Daucus carota): Changes in carotenoid gene expression and carotenoid accumulation in the parasitic weed Phelipanche aegyptiaca and its host
Autor: | Mwafaq Ibdah, Jackline Abu Nassar, Bhagwat Nawade, Dorothea Tholl, Mosaab Yahyaa, Sewar Emran, Hanan Eizenberg |
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Rok vydání: | 2020 |
Předmět: |
cis-trans-Isomerases
0106 biological sciences 0301 basic medicine Molecular biology Tubercle Plant Weeds lcsh:Medicine Strigolactone medicine.disease_cause Biochemistry Plant Roots 01 natural sciences Article Dioxygenases 03 medical and health sciences Orobanchaceae Gene Expression Regulation Plant Infestation Botany medicine lcsh:Science Carotenoid Chromatography High Pressure Liquid Plant Proteins chemistry.chemical_classification Multidisciplinary Phytoene synthase biology organic chemicals lcsh:R food and beverages biology.organism_classification Carotenoids Daucus carota Obligate parasite 030104 developmental biology chemistry Geranylgeranyl-Diphosphate Geranylgeranyltransferase Apocarotenoid biology.protein lcsh:Q Plant sciences 010606 plant biology & botany |
Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | Carotenogenesis has been intensively studied in carrot roots, and transcriptional regulation is thought to be the major factor in carotenoid accumulation in these organs. However, little is known about the transcriptional regulation of carotenoid biosynthetic genes concerning carotenoid accumulation during infestation by the obligate parasite Phelipanche aegyptiaca. HPLC analysis revealed a decrease in carotenoid levels of the different carrot cultivars when parasitized by P. aegyptiaca. Besides, we isolated and analyzed P. aegyptiaca tubercles parasitizing the various carrot root cultivars and show that they accumulate different carotenoids compared to those in non-infested carrot roots. Expression analysis of PHYTOENE SYNTHASE (PSY1) and CAROTENOID ISOMERASE (CRTISO) as well as the strigolactone apocarotenoid biosynthetic genes DWARF27 (D27), CAROTENOID CLEAVAGE DIOXYGENASE 7 (CCD7) and CCD8 revealed that their transcript levels showed significant variation in P. aegyptiaca infested carrot roots. After parasite infestation, the expression of these genes was strongly reduced, as were the carotenoid levels and this was more pronounced in the uncommon non-orange varieties. We also analyzed the parasite genes encoding D27, CCD7 and CCD8 and show that they are expressed in tubercles. This raises important questions of whether the parasite produces its carotenoids and apocarotenoids including strigolactones and whether the latter might have a role in tubercle development. |
Databáze: | OpenAIRE |
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