Contribution of the TGFB1 Gene to Myocardial Infarction Susceptibility
| Autor: | R. M. Barsova, B. V. Titov, N. A. Matveeva, A. V. Favorov, I. N. Rybalkin, T. N. Vlasik, E. M. Tararak, T. S. Sukhinina, R. M. Shahnovich, M. Ya. Ruda, O. O. Favorova |
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| Rok vydání: | 2012 |
| Předmět: |
Genetics
medicine.medical_specialty Linkage disequilibrium Infarction Single-nucleotide polymorphism Odds ratio Biology medicine.disease Biochemistry Gastroenterology Confidence interval Internal medicine Genotype medicine Molecular Medicine Myocardial infarction Allele Molecular Biology Biotechnology |
| Zdroj: | Acta Naturae. 4:74-79 |
| ISSN: | 2075-8251 |
| DOI: | 10.32607/20758251-2012-4-2-74-79 |
| Popis: | Carriage frequencies of alleles and genotypes of the TGFB1 gene polymorphous loci -509C>T (rs1800469), 869T>C (rs1982073), 915G>C (rs1800471), which affect the level of cytokine TGF-β1 production, were analyzed in the patients of Russian ethnic descent with myocardial infarction (MI) (406 cases) and in the control group of the same ethnic descent (198 controls). Significant association with MI was observed in car- riage frequencies of the allele TGFB1*-509T (p=0.046, OR =1.45, 95% CI: 1.02-2.06), genotypes TGFB1*869T/T (p=0.0024, OR =1.75, 95% CI: 1.22-2.51), and TGFB1*915G/G (p=0.048, OR=1.76, 95% CI: 1.05-2.97). Linkage disequilibrium analysis for these SNPs has shown that the associations revealed can be considered to be inde- pendent. A complex analysis of MI association with combinations of alleles/genotypes of said SNPs indicates their cumulative effect. An analysis of susceptibility to early-onset MI (≤ 50 years old) revealed a positive asso- ciation of the allele TGFB1*-509T (p=0.002, OR=2.24, 95% CI: 1.35-3.71) and genotype TGFB1*869T/T (p=0.008, OR=1.93, 95% CI: 1.18-3.15), as well as their additivity. An analysis of susceptibility to recurrent MI revealed an association of the genotype TGFB1*-509T/T (p=0.0078, OR=2.60, 95% CI: 1.28-5.28). The results obtained indi- cate the important role of the TGFB1 gene in susceptibility to MI, including early-onset and recurrent MI, in Russians. KEYWORDS myocardial infarction; Russians; genes; allelic polymorphism; transforming growth factor β1; TGFB1; APSampler. ABBREVIATIONS dNTP - deoxynucleoside triphosphate; LD - linkage disequilibrium; SD - standard deviation; SNP - single nucleotide polymorphism; TGF-β1 - transforming growth factor beta 1; TGFB1 - gene encod- ing transforming growth factor beta 1; CI - confidence interval; IHD - ischemic heart disease; MI - myocar- dial infarction; OR - odds ratio; PCR - polymerase chain reaction; PCR-SSP - polymerase chain reaction with sequence-specific primers; CVD - cardiovascular diseases. |
| Databáze: | OpenAIRE |
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