| Popis: |
BACKGROUND The molecular pathogenesis of endometrial cancer is not yet completely understood, preventing the development of successful therapies. Here, we determine the effect of CYPB on the growth of endometrial cancer. METHODS In this study, we examined the expression of CYPB in endometrial cancer tissues using immunohistochemistry. CYPB silencing in the human cell line HEC-1-B was used to evaluate the role of CYPB in the malignant phenotype of endometrial tumor cells, while CCK-8 and colony formation assays were performed to assess its effect on tumor cell proliferation. Furthermore, microarray analysis was carried out to compare the global mRNA expression profile between the normal and CYPB-knockdown cell. Gene ontology and KEGG pathway enrichment analysis were performed to determine the potential function of different expressed genes related to CYPB. RESULTS We found that CYPB was upregulated in endometrial cancer, while cells with suppressed expression of CYPB exhibited markedly reduced migration. We identified 1536 differentially expressed genes (onefold change, p< 0.05), among which 652 genes were upregulated and 884 genes were downregulated, and most of them were enriched in cell cycle, glycosphingolipid biosynthesis, adherens junctions, and metabolism pathways. CONCLUSIONS The results of our study suggest that CYPB may serve as a novel regulator of endometrial cell proliferation, thus representing a novel target for gene-targeted endometrial therapy. |
