Comparison of different intracellular cryoprotectants on the solid surface vitrification of red‐rumped agouti ( Dasyprocta Leporina Lichtenstein, 1823) ovarian tissue
| Autor: | Samara Sandy Jeronimo Moreira, Alexandre Rodrigues Silva, Lívia Batista Campos, Moacir Franco de Oliveira, Carla Michelle P. de Souza, Andréia Maria da Silva, Gabriela Liberalino Lima, Erica C.G. Praxedes |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Ethylene Glycol
Cryoprotectant Ovarian Cortex Cell Survival Ovary Dasyproctidae Andrology 03 medical and health sciences Follicle chemistry.chemical_compound Cryoprotective Agents 0302 clinical medicine Endocrinology Ovarian Follicle medicine Animals Dimethyl Sulfoxide Vitrification Cryopreservation 030219 obstetrics & reproductive medicine biology Dimethyl sulfoxide 0402 animal and dairy science 04 agricultural and veterinary sciences biology.organism_classification 040201 dairy & animal science Dasyprocta leporina medicine.anatomical_structure chemistry Female Animal Science and Zoology Trypan blue DNA Damage Biotechnology |
| Zdroj: | Reproduction in Domestic Animals. 55:154-161 |
| ISSN: | 1439-0531 0936-6768 |
| DOI: | 10.1111/rda.13600 |
| Popis: | In contributing to the conservation of wild rodents, the aim of this study was to evaluate the use of distinct cryoprotectants, separately or in combination, for solid surface vitrification (SSV) of red-rumped agouti ovarian tissue. Ovarian cortex from nine females was recovered and fragmented. Fresh fragments (control) were used to analyse the pre-antral follicle (PF) morphology using a histologic procedure, viability using the Trypan blue test, cell proliferation by counting the argyrophilic nucleolar organizing regions (Ag-NORs technique) and DNA integrity using the TUNEL assay. The remaining fragments were vitrified using SSV method with 3 M or 6 M ethylene glycol (EG) or dimethyl sulfoxide (DMSO), or in combination (3 M EG/3 M DMSO), and further evaluated as reported for the fresh samples. All cryoprotectants were effective at preserving PFs morphology compared to the control group (80.7 ± 5.21%), except 6 M EG and 3 M DMSO that provoked a significant (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text