Evaluation of Fibroblast Viability Seeded on Acellular Human Amniotic Membrane
| Autor: | Mojtaba Memariani, Alireza Shoae-Hassani, Maryam Kabir-Salmani, Fahimeh Abdollahimajd, Hamed Memariani, Hamideh Moravvej |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Article Subject Cell Survival Cell Culture Techniques General Biochemistry Genetics and Molecular Biology Andrology 030207 dermatology & venereal diseases 03 medical and health sciences chemistry.chemical_compound Foreskin 0302 clinical medicine medicine Humans MTT assay Amnion Propidium iodide DAPI Fibroblast Tissue Scaffolds General Immunology and Microbiology virus diseases food and beverages General Medicine Fibroblasts Staining 030104 developmental biology medicine.anatomical_structure chemistry Cell culture Medicine Wound healing Research Article |
| Zdroj: | BioMed Research International, Vol 2021 (2021) BioMed Research International |
| ISSN: | 2314-6141 2314-6133 |
| DOI: | 10.1155/2021/5597758 |
| Popis: | Background. Investigating the viability and proliferative rates of fibroblast cells on human amniotic membrane (HAM) as a scaffold will be an important subject for further research. The aim of this study was to assess the fibroblast viability seeded on acellular HAM, since foreskin neonatal allogenic fibroblasts seeded on HAM accelerate the wound healing process. Methods. Fibroblasts were retrieved from the foreskin of a genetically healthy male infant, and we exploited AM of healthy term neonates to prepare the amniotic scaffold for fibroblast transfer. After cell culture, preparation of acellular HAM, and seeding of cells on HAM based on the protocol, different methods including 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 4 ′ ,6-Diamidino-2-phenylindole dihydrochloride (DAPI), and propidium iodide (PI) staining were employed for assessment of fibroblast viability on HAM. Results. Based on the results obtained from the DAPI and PI staining, the percentage of viable cells in the former staining was clearly higher than that of the dead cells in the latter one. The results of DAPI and PI staining were in accordance with the findings of MTT assay, confirming that fibroblasts were viable and even proliferate on HAM. Conclusion. Our findings showed the viability of fibroblasts seeded on the acellular HAM using MTT assay, DAPI, and PI staining; however, this study had some limitations. It would be an interesting subject for future research to compare the viability and proliferation rate of fibroblasts seeded on both cellular and acellular HAM. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|