Effect of strontium substitution on the material properties and osteogenic potential of 3D powder printed magnesium phosphate scaffolds
| Autor: | Elke Vorndran, Emilie März, Claus Moseke, Andrea Ewald, Carina Blum, Susanne Meininger, Kerstin Spatz |
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| Rok vydání: | 2019 |
| Předmět: |
Calcium Phosphates
Ceramics Materials science Compressive Strength Strontium carbonate Carbonates Magnesium Compounds chemistry.chemical_element Biocompatible Materials Bioengineering 02 engineering and technology Calcium 010402 general chemistry 01 natural sciences Bone and Bones Phosphates Biomaterials chemistry.chemical_compound Osteogenesis Cell Line Tumor Humans Amorphous calcium phosphate Magnesium phosphate Strontium Osteoblasts Magnesium Cell Differentiation 021001 nanoscience & nanotechnology Phosphate 0104 chemical sciences chemistry Mechanics of Materials Struvite Printing Three-Dimensional Powders 0210 nano-technology Nuclear chemistry |
| Zdroj: | Materials Science and Engineering: C. 98:1145-1158 |
| ISSN: | 0928-4931 |
| DOI: | 10.1016/j.msec.2019.01.053 |
| Popis: | 3D powder printing is a versatile method for the fabrication of individual bone implants and was used for the processing of in vivo degradable ceramic scaffolds based on ammonium magnesium phosphate hexahydrate (struvite). In this study, synergetic effects could be achieved by the substitution of magnesium phosphate cements with strontium carbonate. This substitution resulted in 8.2 wt%, 16.4 wt%, and 24.6 wt% Sr 2+ doped scaffolds, with a 1.9–3.1 times increased radiopacity compared to pure struvite. The maximal compressive strength of (16.1 ± 1.1) MPa found for strontium substituted magnesium phosphate was in the range of cancelleous bone, which makes these 3D printed structures suitable for medical application in low-load-bearing bone areas. In an ion release study over a course of 18 days, the release of strontium, magnesium, calcium, and phosphate ions from scaffolds was analyzed by means of inductively coupled plasma mass spectrometry. Independent of the scaffold composition the Mg 2+ concentrations (83–499 mg/l) continuously increased in the cell media. The Sr 2+ release varied between 4.3 μg/day and 15.1 μg/day per g scaffold, corresponding to a Sr 2+ concentration in media between 1.14 mg/l and 7.24 mg/l. Moreover, decreasing calcium and phosphate concentrations indicated the precipitation of an amorphous calcium phosphate phase. The superior osteogenic properties of strontium substituted magnesium phosphate, e.g. the increase of osteoblast activity and cell number and the simultaneous suppression of osteoclast differentiation could be verified in vitro by means of WST-assay, TRAP-staining, and SEM imaging. |
| Databáze: | OpenAIRE |
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