The nucleotide sequence of Euglena cytoplasmic phenylalanine transfer RNA. Evidence for possible classifications of Euglena among the animal rather than the plant kingdom
| Autor: | Joyce E. Heckman, W E Barnett, Simon H. Chang, L I Hecker, C. K. Brum, Uttam L. RajBhandary, M. Silberklang, J. J. Schnabel |
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| Jazyk: | angličtina |
| Rok vydání: | 1981 |
| Předmět: |
Euglena gracilis
ved/biology.organism_classification_rank.species Phenylalanine RNA Transfer Amino Acyl Euglena Ribonucleases Species Specificity Genetics Animals Nucleotide Ribonuclease T1 Pancreas chemistry.chemical_classification biology Base Sequence ved/biology Nucleic acid sequence biology.organism_classification Biochemistry chemistry Cytoplasm Transfer RNA Nucleic Acid Conformation Research Article |
| Popis: | The nucleotide sequence of cytoplasmic phenylalanine tRNA from Euglena gracilis has been elucidated using procedures described previously for the corresponding chloroplastic tRNA [Cell, 9, 717 (1976)]. The sequence is: pG-C-C-G-A-C-U-U-A-m(2)G-C-U-Cm-A-G-D-D-G-G-G-A-G-A-G-C-m(2)2G-psi-psi-A-G-A-Cm -U-Gm-A-A-Y-A-psi-C-U-A-A-A-G-m(7)G-U-C-*C-C-U-G-G-T-psi-C-G-m(1)A-U-C-C-C-G-G- G-A-G-psi-C-G-G-C-A-C-C-A. Like other tRNA Phes thus far sequenced, this tRNA has a chain length of 76 nucleotides. The sequence of E. gracilis cytoplasmic tRNA Phe is quite different (27 nucleotides out of 76 different) from that of the corresponding chloroplastic tRNA but is surprisingly similar (72 out of 76 nucleotides identical) to that of tRNA Phe from mammalian cytoplasm. This extent of sequence homology even exceeds that found between E. gracilis and wheat germ cytoplasmic tRNA Phe. These findings raise interesting questions on the evolution of tRNAs and the taxonomy of Euglena. |
| Databáze: | OpenAIRE |
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