A spot test for catechol 2:3-oxygenase in bacteria
| Autor: | Eileen S. Pankhurst |
|---|---|
| Rok vydání: | 1965 |
| Předmět: |
Catechol
Oxygenase Bacteriological Techniques Gram-negative bacteria Chromatography biology Positive reaction Catechols In Vitro Techniques biology.organism_classification Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound chemistry Biochemistry Yellow colour Oxygenases Phenol Nutrient agar Bacteria |
| Zdroj: | The Journal of applied bacteriology. 28(2) |
| ISSN: | 0021-8847 |
| Popis: | A simple and quickly performed colour test is described, in which organisms containing catechol 2:3-oxygenase (meta-pyrocatechase) produce from catechol a yellow coloured intermediate, α-hydroxymuconic semialdehyde. The yellow colour is produced most consistently, rapidly and intensely by 2–4 day old cultures from 2·7–27 mM solutions of catechol. The purple-brown colour produced by some organisms is discussed. Using growth from nutrient agar plates, the test was applied to cultures of 239 Gram negative bacteria; all of the 64 cultures which gave a positive result had been in contact previously with aromatic substrates and many of them could oxidize phenol. The significance of a positive reaction is discussed briefly. |
| Databáze: | OpenAIRE |
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