Functional expression of P2Y purinoceptor in Xenopus oocyte injected with brain mRNA
| Autor: | Franck Fournier, P. Guilbault, Thibault Collin, Eric Honoré, Joël Nargeot |
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| Rok vydání: | 1991 |
| Předmět: |
Physiology
Xenopus Clinical Biochemistry Guinea Pigs Injections Adenosine Triphosphate Chlorides Physiology (medical) Gene expression medicine Extracellular Animals RNA Messenger Receptor biology Purinergic receptor Receptors Purinergic Brain biology.organism_classification Oocyte Molecular biology Cell biology Electrophysiology medicine.anatomical_structure Chloride channel Oocytes Calcium Calcium Channels Intracellular |
| Zdroj: | Pflugers Archiv : European journal of physiology. 418(5) |
| ISSN: | 0031-6768 |
| Popis: | Xenopus oocytes injected with embryonic guinea-pig brain mRNA expressed functional P2Y purinoceptors. Extracellular ATP stimulated in a dose-dependent manner a delayed Ca(2+)-dependent Cl- current component. Analysis of the interactions of ATP with compounds that affect Ca2+ fluxes through the plasma membrane or Ca2+ release from internal stores indicates that ATP raises [Ca2+]i by a mechanism that involves activation of voltage-dependent Ca2+ channels, which leads to influx of extracellular Ca2+ into the cells, as well as release of Ca2+ from intracellular stores. Since this phenomenon was not found in control oocytes, it is suggested that brain mRNA encoded for a newly synthesized Ca(2+)-release process stimulated by purinoceptor activation. This mechanism could be largely involved in the short-term regulation of intracellular Ca2+ level involved in ATP neuromodulation functions. |
| Databáze: | OpenAIRE |
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