Acetylation-Dependent Recruitment of the FACT Complex and Its Role in Regulating Pol II Occupancy Genome-Wide in Saccharomyces cerevisiae
| Autor: | Rakesh Pathak, Chhabi K. Govind, Sarah Adamczyk, Olivia Schimmel, Priyanka Singh, Sudha Ananthakrishnan |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Saccharomyces cerevisiae Proteins Transcription Genetic Saccharomyces cerevisiae RNA polymerase II Investigations Histones 03 medical and health sciences Transcription (biology) Gene Expression Regulation Fungal Genetics Gene biology Arginase Chemistry Alcohol Dehydrogenase High Mobility Group Proteins Acetylation FACT complex biology.organism_classification Chromatin Cell biology DNA-Binding Proteins 030104 developmental biology Histone biology.protein RNA Polymerase II Transcriptional Elongation Factors |
| Popis: | Histone chaperones, chromatin remodelers, and histone modifying complexes play a critical role in alleviating the nucleosomal barrier for DNA-dependent processes. Here, we have examined the role of two highly conserved yeast (Saccharomyces cerevisiae) histone chaperones, FACT and Spt6, in regulating transcription. We show that the H3 tail contributes to the recruitment of FACT to coding sequences in a manner dependent on acetylation. We found that deleting a H3 HAT Gcn5 or mutating lysines on the H3 tail impairs FACT recruitment atADH1andARG1genes. However, deleting the H4 tail or mutating the H4 lysines failed to dampen FACT occupancy in coding regions. Additionally, we show that FACT-depletion reduces Pol II occupancy in the 5’ ends genome-wide. In contrast, Spt6-depletion leads to reduction in Pol II occupancy towards the 3’ end, in a manner dependent on the gene-length. Severe transcription and histone eviction defects were also observed in a strain that was impaired for Spt6 recruitment (spt6Δ202) and depleted of FACT. Importantly, the severity of the defect strongly correlated with WT Pol II occupancies at these genes, indicating critical roles of Spt6 and Spt16 in promoting high-level transcription. Collectively, our results show that both FACT and Spt6 are important for transcription globally and may participate during different stages of transcription. |
| Databáze: | OpenAIRE |
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