Peach Fruit Development: A Comparative Proteomic Study Between Endocarp and Mesocarp at Very Early Stages Underpins the Main Differential Biochemical Processes Between These Tissues
| Autor: | María V. Lara, Gabriela Leticia Müller, María F. Drincovich, Carlos E. Rodriguez, Claudia A. Bustamante, Claudio Olaf Budde |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Protein metabolism Plant Science Biological Development 01 natural sciences purl.org/becyt/ford/1 [https] chemistry.chemical_compound Prunus Protein biosynthesis Desarrollo del Fruto Lignificación β–cyanoalanine synthase Original Research chemistry.chemical_classification Fruto Durazno asparagine Amino acid Biochemistry ASPARAGINE lignification Β–CYANOALANINE HYDRATASE β–cyanoalanine hydratase Prunus pérsica Desarrollo Biológico Asparagina PRUNUS PERSICA lcsh:Plant culture Biology Photosynthesis MESOCARP 03 medical and health sciences ENDOCARP FRUIT DEVELOPMENT Mesocarpio LIGNIFICATION lcsh:SB1-1110 Β–CYANOALANINE SYNTHASE purl.org/becyt/ford/1.6 [https] Prunus persica Catabolism β–cianoalanina sintasa Metabolism Endocarpio Metabolic pathway 030104 developmental biology chemistry Peaches Fruit β–cianoalanina hydratasa mesocarp fruit development endocarp 010606 plant biology & botany |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Frontiers in Plant Science Frontiers in Plant Science 10 : 715 (june 2019) INTA Digital (INTA) Instituto Nacional de Tecnología Agropecuaria instacron:INTA Frontiers in Plant Science, Vol 10 (2019) RepHipUNR (UNR) Universidad Nacional de Rosario instacron:UNR |
| DOI: | 10.3389/fpls.2019.00715/full |
| Popis: | Peach (Prunus persica) is an important economically temperate fruit. The development follows double sigmoid curve with four phases (S1–S4). We centered our work in the early development. In addition to S1, we studied the very early stage (E) characterized by the lag zone of the exponential growing phase S1, and the second stage (S2) when the pit starts hardening. “Dixiland” peach fruit were collected at 9 (E), 29 (S1), and 53 (S2) days after flowering (DAF) and endocarp and mesocarp were separated. There was a pronounced decrease in total protein content along development in both tissues. Quantitative proteomic allowed the identification of changes in protein profiles across development and revealed the main biochemical pathways sustaining tissue differentiation. Protein metabolism was the category most represented among differentially proteins in all tissues and stages. The decrease in protein synthesis machinery observed during development would be responsible of the protein fall, rather than a proteolytic process; and reduced protein synthesis during early development would reroute cell resources to lignin biosynthesis. These changes were accompanied by net decrease in total amino acids in E1–S1 and increase in S1–S2 transitions. Amino acid profiling, showed Asn parallels this trend. Concerted changes in Asn and in enzymes involved in its metabolism reveal that increased synthesis and decreased catabolism of Asn may conduct to an Asn increase during very early development and that the β-Cyano-Alanine synthase/β-Cyano-Alanine hydratase could be the pathway for Asn synthesis in “Dixiland” peach fruit. Additionally, photosynthetic machinery decays during early development in mesocarp and endocarp. Proteins related to photosynthesis are found to a higher extent in mesocarp than in endocarp. We conclude mesocarpic photosynthesis is possible to occur early on the development, first providing both carbon and reductive power and latter only reductive power. Together with proteomic, histological tests and anatomical analysis help to provide information about changes and differences in cells and cell-walls in both tissues. Collectively, this work represents the first approach in building protein databases during peach fruit development focusing on endocarp and mesocarp tissues and provides novel insights into the biology of peach fruit development preceding pit hardening. Fil: Rodriguez, Carlos Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Bustamante, Claudia Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Norte. Estación Experimental Agropecuaria San Pedro; Argentina Fil: Müller, Gabriela Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Drincovich, Maria Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Lara, Maria Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina |
| Databáze: | OpenAIRE |
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