Sex-Specific Effects of Dietary Methionine Restriction on the Intestinal Microbiome
| Autor: | Stepan Melnyk, Isabelle R. Miousse, Katherine F. Wallis |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Rikenellaceae Physiology microbiome lcsh:TX341-641 Article 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Protein methylation Animals Microbiome Bacteroidaceae methionine Sex Characteristics Nutrition and Dietetics Methionine biology Bacteria Methylation biology.organism_classification Bacteroidales Gastrointestinal Microbiome Mice Inbred C57BL 030104 developmental biology chemistry 030220 oncology & carcinogenesis Intestinal Microbiome Female methylation diet lcsh:Nutrition. Foods and food supply Food Science |
| Zdroj: | Nutrients Nutrients, Vol 12, Iss 3, p 781 (2020) Volume 12 Issue 3 |
| ISSN: | 2072-6643 |
| Popis: | Dietary methionine restriction is associated with improved health outcomes and an increase in lifespan in animal models. We have previously shown that an increase in dietary methionine induces alteration in the intestinal microbiome. The composition of the intestinal microbiota is a determinant of health and we, therefore, hypothesized that dietary methionine restriction would also induce changes in the murine microbiome. After one month on a methionine-restricted diet, five-month-old male and female C57BL/6 mice had decreased levels of serum methionine, without changes in body weight. We identified a decrease in the hepatic methylation status of animals fed a methionine-restricted diet compared to controls. This decrease was not associated with changes in DNA or protein methylation in the liver. In males, we saw an increase in families Bacteroidaceae and Verrucoccaceae (mostly A. mucinophila) and a decrease in Rumminococcaceae in animals fed a methionine-restricted diet compared to controls. In females, Bacteroidales family S24-7 was increased two-fold, while families Bacteroidaceae, Verrucoccaceae, Rumminococcaceae, and Rikenellaceae were decreased compared to controls. In summary, feeding a methionine-restricted diet for one month was associated with significant and sex-specific changes in the intestinal microbiome. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|