Type 4 OFF cone bipolar cells of the mouse retina express calsenilin and contact cones as well as rods
| Autor: | Heinz Wässle, Johann Helmut Brandstätter, Dana Specht, Nikhat F. Zaidi, Sriparna Majumdar, Silke Haverkamp, Wilma Wasco, Susanne tom Dieck |
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| Rok vydání: | 2007 |
| Předmět: |
Genetically modified mouse
Retinal Bipolar Cells Cell type genetic structures Outer plexiform layer Nerve Tissue Proteins Biology Antibodies Retina Mice chemistry.chemical_compound Retinal Rod Photoreceptor Cells Neural Pathways mental disorders medicine Animals Axon Microscopy Immunoelectron Outer nuclear layer General Neuroscience Kv Channel-Interacting Proteins Retinal Dendrites Inner plexiform layer Mice Mutant Strains Cell biology Mice Inbred C57BL medicine.anatomical_structure chemistry Calsenilin Synapses Retinal Cone Photoreceptor Cells sense organs Neuroscience |
| Zdroj: | The Journal of Comparative Neurology. 507:1087-1101 |
| ISSN: | 1096-9861 0021-9967 |
| DOI: | 10.1002/cne.21612 |
| Popis: | Immunocytochemical discrimination of distinct bipolar cell types in the mouse retina is a prerequisite for analyzing retinal circuitry in wild-type and transgenic mice. Here we demonstrate that among the more than 10 anatomically defined mouse bipolar cell types, type 4 bipolar cells are specifically recognized by anti-calsenilin antibodies. Axon terminals in the inner plexiform layer are not readily identifiable because calsenilin is also expressed in a subset of amacrine and ganglion cells. In contrast, in the outer plexiform layer calsenilin immunoreactivity allows the analysis of photoreceptor to type 4 bipolar cell contacts. A dense plexus of calsenilin-positive dendrites makes several basal contacts at cone pedicles. An individual calsenilin-positive bipolar cell contacts five to seven cones. In addition, some calsenilin-positive dendrites contact rod photoreceptors. On average we counted 10 rod spherule contacts per type 4 bipolar cell, and approximately 10% of rods contacted type 4 bipolar cells. We suggest that type 4 bipolar cells, together with the recently described type 3a and b cells, provide an alternative and direct route from rods to OFF cone bipolar cells. In the Bassoon ΔEx4/5 mouse, a mouse mutant that shows extensive remodeling of the rod system including sprouting of horizontal and rod bipolar cells into the outer nuclear layer due to impaired synaptic transmission, we found that in addition mixed-input (type 3 and 4) OFF bipolar cells sprout to ectopic sites. In contrast, true cone-selective type 1 and 2 OFF cone bipolar cells did not show sprouting in the Bassoon mouse mutant. J. Comp. Neurol. 507: 1087–1101, 2008. © 2007 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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