Moloney murine leukemia virus integrase and reverse transcriptase interact with PML proteins
| Autor: | Hiroshi Higuchi, Hidenori Kaneoka, Yuuki Okino, Yujin Inayoshi, Shinji Iijima, Akiko Honkawa, Shunsuke Kidani, Ken-ichi Nishijima, Katsuhide Miyake, Yusuke Kojima |
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| Rok vydání: | 2012 |
| Předmět: |
Small interfering RNA
viruses SUMO-1 Protein SUMO protein Promyelocytic Leukemia Protein Biochemistry Mice Promyelocytic leukemia protein Retrovirus Complementary DNA Murine leukemia virus Animals Humans Immunoprecipitation Molecular Biology Integrases biology Chemistry Tumor Suppressor Proteins Nuclear Proteins virus diseases RNA-Directed DNA Polymerase General Medicine biology.organism_classification Virology Cell Nucleus Structures Reverse transcriptase Integrase Cell biology Gene Knockdown Techniques Host-Pathogen Interactions NIH 3T3 Cells Small Ubiquitin-Related Modifier Proteins biology.protein Moloney murine leukemia virus HeLa Cells Transcription Factors |
| Zdroj: | The Journal of Biochemistry. 152:161-169 |
| ISSN: | 1756-2651 0021-924X |
| DOI: | 10.1093/jb/mvs063 |
| Popis: | Pull-down assay and co-immunoprecipitation of cell extracts in which the integrase or reverse transcriptase of Moloney murine leukemia virus was transiently expressed showed that both enzymes interacted with PML proteins. In infected cells, interaction between the integrase and PML was also observed. Transient expression of PIASy and SUMO proteins facilitated SUMOylation of the integrase but had no apparent effects on the interaction with PML. A FLAG-tagged integrase co-localized with PML protein possibly in the PML body. Knockdown of PML by small interfering RNA resulted in reduced viral cDNA levels and integration efficiency. This suggested that PML proteins activated reverse transcription. |
| Databáze: | OpenAIRE |
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