Acidic pH triggers the phosphorylation of the response regulator NtrX in alphaproteobacteria

Autor:	Ignacio Fernandez, Gabriela Sycz, Mariela del Carmen Carrica, Fernando Alberto Goldbaum
Jazyk:	angličtina
Rok vydání:	2018
Předmět:	0301 basic medicine Ntrx acidic ph Brucella abortus Gene Expression lcsh:Medicine Artificial Gene Amplification and Extension Pathology and Laboratory Medicine Biochemistry Polymerase Chain Reaction Caulobacter crescentus Gene expression Medicine and Health Sciences Transcriptional regulation Phosphorylation Post-Translational Modification Promoter Regions Genetic lcsh:Science Multidisciplinary biology Chemistry phosphorylation Transcriptional Control Temperature purl.org/becyt/ford/3.1 [https] Hydrogen-Ion Concentration Bioquímica y Biología Molecular Bacterial Pathogens Cell biology Mutant Strains Medicina Básica Experimental Organism Systems Medical Microbiology Physical Sciences Prokaryotic Models purl.org/becyt/ford/3 [https] Pathogens Signal transduction Signal Transduction Research Article CIENCIAS MÉDICAS Y DE LA SALUD response regulator Research and Analysis Methods Regulon Microbiology Phosphates Caulobacter Dephosphorylation 03 medical and health sciences Bacterial Proteins Stress Physiological Genetics Gene Regulation Molecular Biology Techniques Molecular Biology Microbial Pathogens Bacteria lcsh:R Organisms Chemical Compounds Biology and Life Sciences Proteins Gene Expression Regulation Bacterial biology.organism_classification Brucella Response regulator 030104 developmental biology Proteolysis Mutation bacteria lcsh:Q Gene Deletion
Zdroj:	PLoS ONE, Vol 13, Iss 4, p e0194486 (2018) CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET PLoS ONE
ISSN:	1932-6203
Popis:	Many signaling pathways that control cellular development, cell-cycle progression and nutritional versatility have been studied in Caulobacter crescentus. For example, it was suggested that the response regulator NtrX is conditionally essential for this bacterium and that it might be necessary for responding to a signal produced in phosphate-replete minimal medium. However, such signal has not been identified yet and the role of NtrX in C. crescentus biology remains elusive. Here, using wild-type C. crescentus and a strain with a chromosomally myc-tagged ntrX gene, we demonstrate that high concentrations of phosphate (10 mM) regulate ntrX transcription and the abundance of the protein. We also show that the pH of the medium acts as a switch able to regulate the phosphorylation status of NtrX, promoting its phosphorylation under mildly acidic conditions and its dephosphorylation at neutral pH. Moreover, we demonstrate that the ntrX gene is required for survival in environments with low pH and under acidic stress. Finally, we prove that NtrX phosphorylation is also triggered by low pH in Brucella abortus, a pathogenic alphaproteobacterium. Overall, our work contributes to deepen the general knowledge of this system and provides tools to elucidate the NtrX regulon. Fil: Fernandez, Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina Fil: Sycz, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina Fil: Goldbaum, Fernando Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina Fil: Carrica, Mariela del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Databáze:	OpenAIRE
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