Protective effects of components of the Chinese herb grassleaf sweetflag rhizome on PC12 cells incubated with amyloid-beta42
| Autor: | Shanshan Li, Han Wang, Jing Liu, Sumin Tian, Guoying Li, Zi-hao Liang, Zhigang Ruan, Xiao-hui Cheng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
food.ingredient
Amyloid nerve regeneration drugs Chinese herbal Alzheimer′s disease PC12 cells Aβ grassleaf sweetflag rhizome β-asarone eugenol apoptosis neural regeneration Pharmacology Neuroprotection lcsh:RC346-429 chemistry.chemical_compound food Developmental Neuroscience Medicine Asarone lcsh:Neurology. Diseases of the nervous system Messenger RNA Traditional medicine Aβ grassleaf sweetflag rhizome business.industry Alzheimer's disease Rhizome Eugenol chemistry Apoptosis Herb business Research Article |
| Zdroj: | Neural Regeneration Research, Vol 10, Iss 8, Pp 1292-1297 (2015) Neural Regeneration Research |
| ISSN: | 1673-5374 |
| Popis: | The major ingredients of grassleaf sweetflag rhizome are β-asarone and eugenol, which can cross the blood-brain barrier and protect neurons. This study aimed to observe the neuroprotective effects and mechanisms of β-asarone and eugenol, components of the Chinese herb grassleaf sweetflag rhizome, on PC12 cells. First, PC12 cells were cultured with different concentrations (between 1 × 10(-10) M and 1 × 10(-5) M) of β-asarone and eugenol. Survival rates of PC12 cells were not significantly affected. Second, PC12 cells incubated with amyloid-beta42, which reduced cell survival, were cultured under the same conditions (1 × 10(-6) M β-asarone and eugenol). The survival rates of PC12 cells significantly increased, while expression levels of the mRNAs for the pro-apoptotic protein Bax decreased, and those for the anti-apoptotic protein Bcl mRNA increased. In addition, the combination of β-asarone with eugenol achieved better results than either component alone. Our experimental findings indicate that both β-asarone and eugenol protect PC12 cells through inhibiting apoptosis, and that the combination of the two is better than either alone. |
| Databáze: | OpenAIRE |
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