Expression pattern of new genes regulating female sex differentiation and in vitro maturational status of oocytes in pigs

Autor: Michał Nowicki, Małgorzata Bruska, Andrzej Breborowicz, Maurycy Jankowski, Wiesława Kranc, Klaus P. Brüssow, Dorota Bukowska, Maciej Brązert, Piotr Celichowski, Bartosz Kempisty, Leszek Pawelczyk, Michal Jeseta, Maciej Zabel, Marta Dyszkiewicz-Konwińska, Sandra Knap, Katarzyna Ożegowska, Artur Bryja, Paweł Antosik, Mariusz T. Skowronski, Mariusz J. Nawrocki
Rok vydání: 2018
Předmět:
Zdroj: Theriogenology. 121:122-133
ISSN: 0093-691X
DOI: 10.1016/j.theriogenology.2018.08.019
Popis: The processes underlying maturation of mammalian oocytes are considered crucial for the oocytes ability to undergo monospermic fertilization. The same factors of influence are suggested to impact the development of sex associated characteristics, allowing sex differentiation to progress during embryonic growth. The primary aim of the study was to analyze the gene ontology groups involved in regulation of porcine oocytes' response to endogenous stimuli. The results obtained would indicate potential genes influencing sex differentiation. Additionally, they could help to determine new genetic markers, expression profile of which is substantially regulated during porcine oocytes' in vitro maturation. To achieve that, porcine oocytes were collected for analysis before and after in vitro maturation. Pigs were used as they are a readily available model that presents significant similarity to humans in terms of physiology and anatomy. Microarray analysis of oocytes, before and after in vitro maturation was performed and later validated by RT-qPCR. We have particularly detected and analyzed genes belonging to gene ontology groups associated with hormonal stimulation during maturation of the oocytes, that exhibited significant change in expression (fold change ≥ |2|; p 0.05) namely "Female sex differentiation" (CCND2, MMP14, VEGFA, FST, INHBA, NR5A1), "Response to endogenous stimulus" (INSR, ESR1, CCND2, TXNIP, TACR3, MMP14, FOS, AR, EGR2, IGFBP7, TGFBR3, BTG2, PLD1, PHIP, UBE2B) and "Response to estrogen stimulus" (INSR, ESR1, CCND2, IHH, TXNIP, TACR3, MMP14). Some of them were characteristic for just one of the described ontologies, while some belonged into multiple ontological terms. The genes were analyzed, with their relation to the processes of interest explained. Overall, the study provides us with a range of genes that might serve as molecular markers of in vitro maturation associated processes of the oocytes. This knowledge might serve as a reference for further studies and, after further validation, as a potentially useful knowledge in assessment of the oocytes during assisted reproduction processes.
Databáze: OpenAIRE
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